Recombinant Human Transglutaminase 4/TGM4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5760-TG

R&D Systems, part of Bio-Techne
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5760-TG-050
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

AAC50516

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Transglutaminase 4/TGM4 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Transglutaminase 4/TGM4 protein
Met2-Lys684, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.

Predicted Molecular Mass

78 kDa

SDS-PAGE

65-70 kDa, reducing conditions

Activity

Measured by its ability to form CBZ-Gln-Gly-Hydroxamate from CBZ-Gln-Gly and Hydroxylamine.
The specific activity is >50 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5760-TG
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Transglutaminase 4/TGM4

Transglutaminase 4, also known as TGM4, TG4, hTGP, or the prostate-specific transglutaminase, is a member of the transglutaminase family that is exclusively expressed and secreted from the prostate (1, 2). Transglutaminases catalyze the calcium-dependent formation of isopeptide bonds between lysine and glutamine residues (3). In rodents, TGM4 is responsible for the formation of the copulatory plug and sperm antigenicity, but the function in humans is largely unknown (4). The expression of TGM4 is primarily regulated by retinoic acid and secondarily modulated by androgens (5).

References

  1. Dubbink, H.J. et al. (1996) Biochem. J. 315:901.
  2. Dubbink, H.J. et al. (1999) Lab. Invest. 79:141.
  3. Lorand, L. and Graham, R.M. (2003) Nature Rev. Mol. Cell. Biol. 4:140.
  4. Williams-Ashman, H.G. et al. (1972) Proc. Natl. Acad. Sci. USA 69:2322.
  5. Rivera-Gonzalez, G.C. et al. (2012) Nucleic Acids Res. 40:4825.

Alternate Names

Fibrinoligase, TGM4, TGP

Entrez Gene IDs

7047 (Human); 331046 (Mouse); 64679 (Rat)

Gene Symbol

TGM4

UniProt

AAC50516

Additional Transglutaminase 4/TGM4 Products

Product Documents for Recombinant Human Transglutaminase 4/TGM4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Transglutaminase 4/TGM4 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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