Recombinant Human Trypsin 1/PRSS1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3848-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Trypsin 1/PRSS1 protein
Ala16-Ser247, with a C-terminal 10-His tag
Ala16-Ser247, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala16
Predicted Molecular Mass
26 kDa
SDS-PAGE
32 kDa and 36 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
3848-SE
| Formulation | Supplied as a 0.2 μm filtered solution in HCl and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Trypsin 1/PRSS1
Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen (1). Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes (2). Mutations in the PRSS1 gene can cause hereditary pancreatitis (3).
References
- Emi, M. et al. (1986) Gene 41:305.
- Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1483, Academic Press, San Diego.
- Teich, N. et al. (2006) Hum. Mutat. 27:721.
Alternate Names
PRSS1, PTRYI, TRY4, Trygn16, Trypsinogen 1
Gene Symbol
PRSS1
UniProt
Additional Trypsin 1/PRSS1 Products
Product Documents for Recombinant Human Trypsin 1/PRSS1 Protein, CF
Product Specific Notices for Recombinant Human Trypsin 1/PRSS1 Protein, CF
For research use only
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