Recombinant Human Trypsin 2/PRSS2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3586-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Trypsin 2/PRSS2 protein
Ala16-Ser247, with a C-terminal 10-His tag
Ala16-Ser247, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala16
Predicted Molecular Mass
26 kDa
SDS-PAGE
34 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >8,000 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 3586-SE in the following applications:
Formulation, Preparation and Storage
3586-SE
| Formulation | Supplied as a 0.2 μm filtered solution in HCl and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Trypsin 2/PRSS2
Human Trypsin 2, encoded by the PRSS2 gene, is also known as anionic Trypsin (1). Accounting for approximately 1/3 of the total trypsin content in the pancreatic juice, it is one of the trypsins produced in the pancreas. It consists of a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 2 is synthesized as a zymogen and secreted into the duodenal lumen, where it is activated by enteropeptidase (2). It plays a central role in digestion and activating other pro-enzymes. In human pancreatic diseases and chronic alcoholism, it is up-regulated (3).
References
- Emi, M. et al. (1986) Gene. 41:305.
- Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 1483-1488, Academic Press, San Diego.
- Rinderknecht, H. et al. (1979) Gut. 20:886.
Alternate Names
PRSS2, Tesp4, TRY8, Trypsinogen 20
Gene Symbol
PRSS2
UniProt
Additional Trypsin 2/PRSS2 Products
Product Documents for Recombinant Human Trypsin 2/PRSS2 Protein, CF
Product Specific Notices for Recombinant Human Trypsin 2/PRSS2 Protein, CF
For research use only
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