Recombinant Human UbcH5a/UBE2D1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E2-616
Key Product Details
Product Specifications
Source
E. coli-derived human UbcH5a/UBE2D1 protein
Met1 - Met147
Met1 - Met147
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
17 kDa
Activity
Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5a/UBE2D1 concentration of 0.1-1 μM.
Formulation, Preparation and Storage
E2-616
| Formulation | Supplied as a solution in HEPES, NaCl, TCEP and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: UbcH5a/UBE2D1
Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT) (1,2). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively (3). Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins (4). UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53 (1). Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1 (5,6). Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses (7).
References
- Scheffner, M. et al. (1994) Proc. Natl. Acad. Sci. USA 91:8797.
- Gehrke, S. et al. (2003) Blood 101:3288.
- Jensen, J. et al. (1995) J. Biol. Chem. 270:30408.
- Lorick, K. et al. (2005) Methods Enzymol. 398:54.
- Stancovski, I. et al. (1995) Mol. Cell Biol. 15:7106.
- Dynek, J. et al. (2010) EMBO J. 29:4128.
- Vanni, E. et al. (2012) J. Virol. 86:6323.
Alternate Names
E2(17)KB1, UBC4/5, UBC5A, UBE2D1
Gene Symbol
UBE2D1
UniProt
Additional UbcH5a/UBE2D1 Products
Product Documents for Recombinant Human UbcH5a/UBE2D1 Protein, CF
Product Specific Notices for Recombinant Human UbcH5a/UBE2D1 Protein, CF
For research use only
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