Recombinant Human ULBP-4/RAET1E Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6285-UL

R&D Systems, part of Bio-Techne
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6285-UL-050
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Key Product Details

Source

NS0

Accession #

Q8TD07

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ULBP-4/RAET1E Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ULBP-4/RAET1E protein
Human ULBP-4
(Gly30-Asp225)
Accession # Q8TD07		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly30

Predicted Molecular Mass

49 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to induce IFN-gamma secretion in NK-92 human natural killer lymphoma cells.
The ED50 for this effect is typically 2-10 μg/mL.

Formulation, Preparation and Storage

6285-UL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ULBP-4/RAET1E

RAET1E (retinoic acid early transcript 1E), also called ULBP-4 (cytomegalovirus glycoprotein UL16 binding protein 4), Letal (lymphocyte effector cell toxicity activating ligand) and NKG2DL4 (NKG2D ligand 4), is a 40-50 kDa member of the RAET1/ULBP family of cell surface proteins that function as ligands for NKG2D (1‑6). While most family members are GPI-anchored, only RAET1E/ULBP-4 and ULBP-5/RAET1G express a transmembrane form (1, 4, 6, 7). Human RAET1E mRNA encodes 263 amino acids (aa) including a 30 aa signal sequence, a 195 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 15 aa cytoplasmic sequence. A soluble 35 kDa form diverges at aa 208 and is thought to antagonize the transmembrane form (5). Other potential splice variants of 220, 227 and 280 aa are transmembrane proteins (8). Within the ECD, RAET1E shares 34-41% aa sequence identity with family members (1, 7). Rodent NKG2D ligands Rae-1 alpha-epsilon are, like human ULBP and MIB proteins, distantly related to MHC class I proteins, but none of the families share significant sequence identity (2, 4). Low expression of RAET1E mRNA is found in normal tissues, with high expression variably reported in the small intestine (3) and skin (4). Expression is stimulated by TNF-alpha and down‑regulated by retinoic acid (3). RAET1E is abnormally expressed on most colon cancer and some other tumor cell lines and virus-infected peripheral blood cells (3, 6). RAET1E binds and costimulates NKG2D-expressing effector cells including NK cells, NKT cells, gamma delta T cells, and CD8+ alpha beta T cells, activating cytolytic activity and/or cytokine production (3, 4, 7). In some gamma delta T cells, direct RAET1E binding to both TCR gamma delta and NKG2D has been demonstrated (6). RAET1E is also thought to function as a minor histocompatibility antigen in humans (1).

References

  1. Radosavljevic, M. et al. (2002) Genomics 79:114.
  2. Kondo, M. et al. (2010) Immunogenetics 62:441.
  3. Conejo-Garcia, J.R. et al. (2003) Cancer Biol. Ther. 2:446.
  4. Chalupny, N.J. et al. (2003) Biochem. Biophys. Res. Commun. 305:129.
  5. Cao, W. et al. (2007) J. Biol. Chem. 282:18922.
  6. Kong, Y. et al. (2009) Blood 114:310.
  7. Bacon, L. et al. (2004) J. Immunol. 173:1078.
  8. Cao, W. et al. (2008) Int. Immunol. 20:981.

Long Name

UL16 Binding Protein-4

Alternate Names

LETAL, N2DL-4, N2DL4, NKG2DL4, RAET1E, RL-4, ULBP4

Entrez Gene IDs

135250 (Human); 379043 (Mouse); 292461 (Rat)

Gene Symbol

RAET1E

UniProt

Q8TD07 (Human)

Additional ULBP-4/RAET1E Products

Product Documents for Recombinant Human ULBP-4/RAET1E Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human ULBP-4/RAET1E Fc Chimera Protein, CF

For research use only

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