Recombinant Human UPP1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7234-UP

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

Q16831

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Uridine Phosphorylase 1/UPP1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Uridine Phosphorylase 1/UPP1 protein
Ala2-Ala310, with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

35 kDa

SDS-PAGE

32-34 kDa, reducing conditions

Activity

Measured by its ability to catalyze the phosphorolysis of uridine to uracil and ribose-1-phosphate.
The specific activity is >9,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7234-UP
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Uridine Phosphorylase 1/UPP1

Uridine Phophorylase 1 (UPP1) belongs to the family of pentosyltransferases. UPP1 is expressed in the cytosol of most tissues and at elevated levels in many tumors (1, 2). It plays an important role in the pyrimidine salvage pathway through its catalysis of the reversible phosphorolysis of uridine to uracil (1, 2). UPP1 is important for the homeostatic regulation of intracellular and plasma uridine concentrations (3). UPP1 also plays an important role in the activation of 5-fluorouracil, an anti-tumor drug (4). The high level of UPP1 expression in some tumors makes it a potential prognostic factor for some cancers, such as oral squamous cell carcinoma (5).

References

  1. Krenitsky, T. A. et al. (1964) J. Biol. Chem. 239:805.
  2. Cass, C. E. and Paterson, A. R. P. (1972) J. Biol. Chem. 247:3314.
  3. Connolly, G.P. and Duley, J. A. (1999) Trends Pharmacol. Sci. 20:218.
  4. Cao, D. and Pizzorno, G. (2004) Drugs Today (Barc) 40:431.
  5. Miyashita, H. et al. (2002) Cancer 94:2959.

Alternate Names

UDPRASE, UP, UPASE 1, UPP, UrdPase 1

Entrez Gene IDs

7378 (Human); 22271 (Mouse); 289801 (Rat)

Gene Symbol

UPP1

UniProt

Q16831

Additional Uridine Phosphorylase 1/UPP1 Products

Product Documents for Recombinant Human UPP1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human UPP1 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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