Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF Best Seller

R&D Systems, part of Bio-Techne | Catalog # 862-VC

R&D Systems, part of Bio-Techne
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862-VC-100
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Key Product Details

Source

NS0

Accession #

P19320

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VCAM-1/CD106 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human VCAM-1/CD106 protein
Met Human VCAM-1
(Phe25-Glu698)
Accession # P19320		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

101.6 kDa (monomer)

SDS-PAGE

115-130 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
When 5 x 104 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), approximately 90-100% will adhere after 1 hour incubation at RT.
Optimal dilutions should be determined by each laboratory for each application.

Reviewed Applications

Read 1 review rated 5 using 862-VC in the following applications:

Scientific Data Images for Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF

Recombinant Human VCAM‑1/CD106 Fc Chimera Protein SEC-MALS.

Recombinant Human VCAM1/Fc/His (Catalog # 862-VC) has a molecular weight (MW) of 231.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Formulation, Preparation and Storage

862-VC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 week, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

Human VCAM-1 (CD106, INCAM-110) is a cell adhesion molecule and a member of the immunoglobulin superfamily. Alternatively spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 amino acid extracellular segment that includes seven C2-type immunoglobulin domains. VCAM-1 is expressed by activated endothelial cells and certain other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes. VCAM-1 binds to leukocyte integrins alpha4 beta1 (VLA-4) and alpha4 beta7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1:VLA-4/ alpha4 beta7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Gene Symbol

VCAM1

UniProt

P19320

Additional VCAM-1/CD106 Products

Product Documents for Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF

For research use only

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