Recombinant Human VCAM-1/CD106 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10201-VC

R&D Systems, part of Bio-Techne
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10201-VC-100
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Key Product Details

Source

CHO

Accession #

P19320-1

Conjugate

Unconjugated

Applications

Bioactivity

View all VCAM-1/CD106 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VCAM-1/CD106 protein
Phe25-Glu698, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe25

Predicted Molecular Mass

75 kDa

SDS-PAGE

80-110 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
The ED50 for this effect is 0.5-3 μg/mL.

Scientific Data Images for Recombinant Human VCAM-1/CD106 His-tag Protein, CF

Recombinant Human VCAM-1/CD106 His-tag Protein Bioactivity

Recombinant Human VCAM-1/CD106 His-tag Protein Bioactivity

Recombinant Human VCAM-1/CD106 His-tag (Catalog # 10201-VC) supports the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is 0.5-3 μg/mL.
Recombinant Human VCAM-1/CD106 His-tag Protein SDS-PAGE

Recombinant Human VCAM-1/CD106 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human VCAM-1/CD106 His-tag (Catalog # 10201-VC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-110 kDa.

Formulation, Preparation and Storage

10201-VC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

VCAM-1, also known as CD106, is an immunoglobulin (Ig)-like adhesion molecule that is mainly expressed in endothelial cells and other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes (1, 2). It plays a critical role in inflammation by recruiting leukocytes to acute and chronic inflammation sites (3, 4). Alternatively-spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 aa extracellular domain (ECD) that includes seven C2-type immunoglobulin domains, a 22 aa transmembrane segment, and a 19 amino acid (aa) cytoplasmic tail. Within the ECD, human VCAM-1 shares 75% and 76% aa sequence identity with the mouse and rat VCAM-1, respectively. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation (5). ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals, but elevated levels of serum VCAM-1 are indicative of future Atrial Fibrillation incident as well as liver disease (6, 7). Tumor cells use overexpression of VCAM-1 as means of escaping immune surveillance (8).

References

  1. Vonderheide, R.H. et al. (1994) J. Cell Biol. 125:215.
  2. Cybulsky, M.I. et al. (1991) Proc. Natl. Acad. Sci. USA 88:7859.
  3. Luster, A.D. et al. (2005) Nat. Immunol. 6:1182.
  4. Osborn, L. et al. (1989) Cell 59:1203
  5. Langer. H.F. et al. 2009. J Cell Mol Med. 13:1211.
  6. Willeit.K. et al. 2017. JAMA Cardiol. 2:516.
  7. Lo Iacono.O. et al. 2008. Liver Int. 28:1129.
  8. Wu.T.C. et al. 2007. Cancer Research. 67:6003

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Gene Symbol

VCAM1

UniProt

P19320-1

Additional VCAM-1/CD106 Products

Product Documents for Recombinant Human VCAM-1/CD106 His-tag Protein, CF

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Product Specific Notices for Recombinant Human VCAM-1/CD106 His-tag Protein, CF

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