Recombinant Human VCAM-1/CD106 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 809-VR
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human VCAM-1/CD106 protein
Phe25-Glu698, with a substitution of Arg322Gly and an N-terminal Met
Phe25-Glu698, with a substitution of Arg322Gly and an N-terminal Met
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
74 kDa
SDS-PAGE
90-95 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
When 5 x 104 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
When 5 x 104 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% cells will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Reviewed Applications
Read 1 review rated 5 using 809-VR in the following applications:
Formulation, Preparation and Storage
809-VR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol. |
| Reconstitution |
Reconstitute at 1 mg/mL in sterile, deionized water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: VCAM-1/CD106
Human VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN‑ gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha4 beta7.
During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha4 beta7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
UniProt
Additional VCAM-1/CD106 Products
Product Documents for Recombinant Human VCAM-1/CD106 Protein, CF
Product Specific Notices for Recombinant Human VCAM-1/CD106 Protein, CF
For research use only
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