Recombinant Human VCAM-1/CD106 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # ADP5

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
ADP5-050
ADP5-200
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

P19320-1

Conjugate

Unconjugated

Applications

Bioactivity

View all VCAM-1/CD106 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VCAM-1/CD106 protein
Phe25-Glu698 (Arg322Gly)

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

74.1 kDa

SDS-PAGE

93-95 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
When 5 x 104 cells/well are added to human sVCAM-1 coated plates (10 μg/mL with 100 μL/well), >85% will adhere after 1 hour incubation at room temperature.

Formulation, Preparation and Storage

ADP5
Formulation Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Reconstitution
Reconstitute at 1 mg/mL in sterile, deionized water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

Human VCAM-1 (CD106), a member of the Immunoglobulin Superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha4 beta7.During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha4 beta7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Gene Symbol

VCAM1

UniProt

P19320-1

Additional VCAM-1/CD106 Products

Product Documents for Recombinant Human VCAM-1/CD106 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human VCAM-1/CD106 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.