Recombinant Human VE-Cadherin Protein His-tag, CF
R&D Systems, part of Bio-Techne | Catalog # 8440-VC
Key Product Details
Source
HEK293
Accession #
Structure / Form
Non-covalent dimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human VE-Cadherin protein
Asp48-Gln599, with a C-terminal 6-His tag
Asp48-Gln599, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp48
Predicted Molecular Mass
63 kDa
SDS-PAGE
80-94 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D-1b bovine corneal endothelial cells.
The ED50 for this effect is typically 20-100 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
The ED50 for this effect is typically 20-100 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
8440-VC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 250 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: VE-Cadherin
VE-Cadherin is a 125-135 kDa transmembrane glycoprotein that consistis of a 552 amino acid (aa) extracellular domain (ECD) with five Ca++-binding Cadherin domains, a 21 aa transmembrane segment, and a 164 aa cytoplasmic domain (2, 3). Within the ECD, human VE-Cadherin shares approximately 75% aa sequence identity with mouse and rat VE-Cadherin. A 90 kDa portion of the VE-Cadherin ECD can be shed in a metalloproteinase-dependent mechanism and may function as a VE-Cadherin antagonist (4, 5). VE-Cadherin is expressed on the surface of vascular endothelial cells (EC) from early in embryogenesis through adulthood as well as in the placenta, on hematopoietic cell progenitors, and on a subpopulation of hematopoietic stem cells (6-8). It is a major protein component of adherens junctions between cells of the endothelium (9). VE-Cadherin supports the extension and stabilization of existing vascular sprouts (10). VE-Cadherin also regulates or is regulated by VEGF R2, type I and type II TGF-beta receptors, and other endothelial junction proteins such as JAM-C, Claudin-5, and N-Cadherin (11-15). Elevated levels of soluble VE-Cadherin are found in the serum of myocardial infarction, angina pectoris, rheumatoid arthritis, and colorectal cancer patients (16-18).
References
- Giannotta, M. et al. (2013) Dev. Cell 26:441.
- Breviario, F. et al. (1995) Arterioscler. Throm. Vasc. Biol. 15:1229.
- Geyer, H. et al. (1999) Glycobiology 9:915.
- Herren, B. et al. (1998) Mol. Biol. Cell 9:1589.
- Li, H. et al. (2010) Cancer Gene Ther. 17:700.
- Breier, G. et al. (1996) Blood 87:630.
- Ema, M. et al. (2006) Blood 108:4018.
- Oberlin, E. et al. (2010) Blood 116:4444.
- Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.
- Perryn, E.D. et al. (2008) Dev. Biol. 313:545.
- Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
- Orlova, V.V. et al. (2006) J. Exp. Med. 203:2703.
- Taddei, A. et al. (2008) Nat. Cell Biol. 10:923.
- Luo, Y. and G.L. Radice (2005) J. Cell Biol. 169:29.
- Rudini, N. et al. (2008) EMBO J. 27:993.
- Soeki, T. et al. (2004) Circ. J. 68:1.
- Sulkowska, M. et al. (2006) Tumori 92:67.
- Sidibe, A. et al. (2012) Arthritis Rheum. 64:77.
Long Name
Vascular Endothelium Cadherin
Alternate Names
Cadherin-5, CD144, CDH5, VECadherin
Gene Symbol
CDH5
UniProt
Additional VE-Cadherin Products
Product Documents for Recombinant Human VE-Cadherin Protein His-tag, CF
Product Specific Notices for Recombinant Human VE-Cadherin Protein His-tag, CF
For research use only
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