Recombinant Human VEGF-111b Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10036-VE

R&D Systems, part of Bio-Techne
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10036-VE-050
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Key Product Details

Source

E. coli

Accession #

P15692-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VEGF Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human VEGF protein
Ala27-Arg131, followed by SLTRKD (B isoform, Reference 7)

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro28

Predicted Molecular Mass

13 kDa

SDS-PAGE

12 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human VEGF R2/KDR Fc Chimera (Catalog # 357-KD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human VEGF 111b binds with an ED50 of 12-72 ng/mL.

Scientific Data Images for Recombinant Human VEGF-111b Protein, CF

Recombinant Human VEGF-111b Protein Binding Activity

Recombinant Human VEGF-111b Protein Binding Activity

When Recombinant Human VEGF R2/KDR Fc Chimera (Catalog # 357-KD) is immobilized at 2 µg/mL, Recombinant Human VEGF111b (Catalog # 10036-VE) binds with an ED50 of 12-72 ng/mL.
Recombinant Human VEGF-111b Protein SDS-PAGE

Recombinant Human VEGF-111b Protein SDS-PAGE

2 μg/lane of Recombinant Human VEGF 111b was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 12 kDa and 33 kDa, respectively.

Formulation, Preparation and Storage

10036-VE
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (4). Two families of VEGF isoforms are produced through alternative splicing: VEGF xxx (pro-angiogenic) and VEGF xxxb (anti-angiogenic) families that differ by only six amino-acids at the C-terminal end. The B isoform ends with SLTRKD instead of CDKPRR (5). The first verified and widely reported VEGF xxxb family member is VEGF 165b (6). VEGF 111b is a newly discovered member that is induced by UV-B irradiation or by mitomycin C. It doesn't express in normal conditions and has a remarkable resistance to proteolysis (6). Human VEGF 111b shares 87% aa sequence identity with corresponding regions of mouse and rat VEGF. VEGF 111b contains binding domains for VEGF R1 and VEGF R2, however it lacks binding sites for NRP-1 and heparin and cleavage sites for plasmin and matrix metalloproteinases (7, 8). VEGF 111b has been shown to inhibit the proliferation of HUVECs and ovarian cancer cells as well as migration and tube formation of HUVECs (6-7). It has been demonstrated that VEGF 111b functions through VEGF R2 to inhibit the downstream PI3K, Akt, and ERK1/2 signaling pathways (6, 7).

References

  1. Leung, D.W. et al. (1989) Science 246:1306.
  2. Keck, P.J. et al. (1989) Science 246:1309.
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
  4. Robinson, C.J. and Stringer, S.E. (2001) J. Cell. Sci. 114:853.
  5. Nowak, D.G. et al. (2008) J. Cell Sci. 121:3487.
  6. Li, X. et al. (2015). J.Transl. Med.13:164.
  7. Gu, F. et al. (2013) Biochem Biophys Res Commun. 441:18.
  8. Dehghanian, F. and Hojati, Z. (2014) Gene 553:57.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

UniProt

P15692-1

Additional VEGF Products

Product Documents for Recombinant Human VEGF-111b Protein, CF

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Product Specific Notices for Recombinant Human VEGF-111b Protein, CF

For research use only

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