Recombinant Human VHR Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1654-VH
Key Product Details
Product Specifications
Source
E. coli-derived human VHR protein
Ser2-Pro185, with an N-terminal Met and 6-His tag
Ser2-Pro185, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
21 kDa
SDS-PAGE
23 kDa, reducing conditions
Activity
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >100 pmol/min/μg, as measured under the described conditions.
The specific activity is >100 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
1654-VH
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: VHR
Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to dephosphorylate cellular ERK1, ERK2 and JNK, but not p38 (2, 3). Phosphorylation of VHR at tyrosine 138 by ZAP-70 enhances inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor responses (3). The enzymatic mechanism for VHR has been studied in detail (4) and its X-ray crystal structure has been characterized (5). Its well-defined biochemistry has made it useful in screening assays for compounds that inhibit phosphatases (6).
References
- Ishibashi, T. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12170.
- Todd, J.L. et al. (1999) J. Biol. Chem. 274:13271.
- Alonso, A. et al. (2003) Nat. Immunol. 4:44.
- Zhang, Z.-Y. et al. (1995) Biochemistry 34:16088.
- Yuvaniyama, J. et al. (1996) Science 272:1328.
- Ueda, K. et al. (2002) FEBS Lett. 525:48.
Long Name
Vaccinia Virus VH1-related Phosphatase
Alternate Names
DUSP3
Entrez Gene IDs
1845 (Human)
Gene Symbol
DUSP3
UniProt
Additional VHR Products
Product Documents for Recombinant Human VHR Protein, CF
Product Specific Notices for Recombinant Human VHR Protein, CF
For research use only
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