Recombinant Human VIPR2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11003-VR

R&D Systems, part of Bio-Techne
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11003-VR-050
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Key Product Details

Source

HEK293

Accession #

P41587.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VIPR2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human VIPR2 protein
Recombinant Human VIPR2
(Arg26-Val126)
Accession # P41587.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg26

Predicted Molecular Mass

38 kDa

SDS-PAGE

45-60 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Growth Hormone-Releasing Factor is immobilized at 2.00 μg/mL, 100 μL/well, Recombinant Human VIPR2 Fc Chimera binds with an ED50 of 7.00-42.0 μg/mL.

Scientific Data Images for Recombinant Human VIPR2 Fc Chimera Protein, CF

Recombinant Human VIPR2 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human VIPR2 Fc Chimera Protein (Catalog # 11003-VR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-60 kDa and 90-120 kDa, respectively.

Formulation, Preparation and Storage

11003-VR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VIPR2

Vasoactive intestinal polypeptide receptor 2 (VIPR2 or VPAC2) is a transmembrane receptor that belongs to the B class G protein coupled receptor family of proteins (1). Mature, human VIPR2 consists of an extracellular domain (ECD) with a hormone receptor region followed by seven transmembrane regions and a short cytoplasmic domain. The ECD of human VIPR2 shares 90% amino acid sequence identity with both mouse and rat VIPR2. Human VIPR2 is expressed predominantly in skeletal muscle, and to a lesser extent in the brain, heart, pancreas, and placenta (1). VIPR2 is coupled to a cAMP mediated signal transduction pathway and binds two homologous neuropeptides, vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP), with high affinity (1). VIP plays several roles during neural development, including stimulating neurogenesis, promoting survival of neurons, and assisting in neuron repair (1, 2). Additionally, VIP signaling via VIPR2 has been implicated in regulating immune responses (3). VIPR2 gene duplication and altered signaling of VIP has been associated with the pathogenesis of schizophrenia and may also play a role in autism (4-6). VIPR2 activation has been linked to disruption of cortical neuronal maturation in mice (7). Studies suggest that VIPR2 could be a potential target in the development of novel antipsychotic drugs (5).

References

  1. Lutz, E. M. et al. (1999) FEBS Letters. 458:197.
  2. Hill, J. et al. (2007) Curr. Pharm. Des. 13:1079.
  3. Abad, C. and Var Tan, Y. (2016) J Clin Exp Neuroimmunol 1:104.
  4. Vacic, V. et al. (2011) Nature. 471:499.
  5. Levinson, D. et al. (2011) Ameri. J. Psych. 168:302.
  6. Firouzabadi, S. G. et al. (2017) Molec. Neuobio. 54:7019.
  7. Takeuchi, S. et al. (2020) Front. Neurosci. 14:521.

Long Name

Vasoactive Intestinal Peptide Receptor 2

Alternate Names

PACAPR3, VIP R2, VPAC2, VPCAP2R

Entrez Gene IDs

7434 (Human); 22355 (Mouse); 29555 (Rat)

Gene Symbol

VIPR2

UniProt

P41587.2

Additional VIPR2 Products

Product Documents for Recombinant Human VIPR2 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human VIPR2 Fc Chimera Protein, CF

For research use only

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