Recombinant Human VISTA/B7-H5/PD-1H His Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9057

Biotinylated
R&D Systems, part of Bio-Techne
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AVI9057-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

AAH20568

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all VISTA/B7-H5/PD-1H Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human VISTA/B7-H5/PD-1H protein
Human VISTA/B7-H5/PD-1H
(Phe33-Ala194)
Accession # AAH20568		 HHHHHH  Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe33

Predicted Molecular Mass

21 kDa

SDS-PAGE

35-50 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When serially diluted Biotinylated Recombinant Human VISTA/B7-H5/PD-1H His-tag Avi-tag Protein (Catalog # AVI9057) is immobilized onto a Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human VSIG-3 Fc Chimera (Catalog # 9229-VS) with an ED50 of 75-450 ng/ml.

Scientific Data Images for Recombinant Human VISTA/B7-H5/PD-1H His Avi-tag Protein, CF

Recombinant Human VISTA/B7-H5/PD-1H His Avi-tag Protein Binding Activity

Recombinant Human VISTA/B7-H5/PD-1H His Avi-tag Protein Binding Activity

When serially diluted Biotinylated Recombinant Human VISTA/B7-H5/PD-1H His-tag Avi-tag Protein (Catalog # AVI9057) is immobilized onto an EvenCoat Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) with an ED50 of 75-450 ng/mL.
Recombinant Human VISTA/B7-H5/PD-1H His Avi-tag Protein SDS-PAGE

Recombinant Human VISTA/B7-H5/PD-1H His Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human VISTA/B7-H5/PD-1H His-tag Avi-tag (Catalog # AVI9057) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-50 kDa.

Formulation, Preparation and Storage

AVI9057
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VISTA/B7-H5/PD-1H

Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7-H5, is a 55-65 kDa transmembrane glycoprotein with homology to B7-like immune costimulatory molecules (1, 2). Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively (3). The 30 kDa ECD can be shed by MT1-MMP, with a 25-30 kDa fragment remaining in the membrane (3). Gi24 promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2 (3). Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but is also down-regulated following BMP-4 exposure (4, 5). It binds to BMP-4 directly, and also associates with the type I BMP receptor Activin RIB/ALK-4 (4, 5). Gi24 is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes (6, 7). It is up-regulated in vivo on activated monocytes and dendritic cells (5). Gi24 inhibits CD4+ and CD8+ T cell proliferation, and their production of IL-2 and IFN-gamma (6). Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression (6). In contrast, Gi24 limits disease progression in the autoimmune disease model EAE (6). Gi24 also acts as a co-inhibitory receptor on alloreactive T cells to regulate T cell tolerance (8).

References

  1. Flajnik, M.F. et al. (2012) Immunogenetics 64:571.
  2. Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
  3. Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
  4. Aloia, L. et al. (2010) J. Biol. Chem. 285:7776.
  5. Parisi, S. et al. (2012) FASEB J. 26:3957.
  6. Wang, L. et al. (2011) J. Exp. Med. 208:577.
  7. Ren, G. et al. (2013) PLoS One 8:e65531.
  8. Flies, D.B. et al. (2015) J. Immunol. 194:5294.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Gene Symbol

VSIR

UniProt

AAH20568

Additional VISTA/B7-H5/PD-1H Products

Product Documents for Recombinant Human VISTA/B7-H5/PD-1H His Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human VISTA/B7-H5/PD-1H His Avi-tag Protein, CF

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