Recombinant Human Vitamin D BP His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3778-DB

R&D Systems, part of Bio-Techne
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3778-DB-050
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Key Product Details

Source

NS0

Accession #

P02774

Conjugate

Unconjugated

Applications

Bioactivity

View all Vitamin D BP Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Vitamin D BP protein
Leu17-Leu474 (K436T), with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu17

Predicted Molecular Mass

52 kDa

SDS-PAGE

57-63 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Vitamin D3-BSA Conjugate is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Vitamin D BP binds with an ED50 of 2-10 μg/mL.

Scientific Data Images for Recombinant Human Vitamin D BP His-tag Protein, CF

Recombinant Human Vitamin D BP His-tag Protein SDS-PAGE

Recombinant Human Vitamin D BP His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Vitamin D BP was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-63 kDa and 45-50 kDa, respectively.

Formulation, Preparation and Storage

3778-DB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Vitamin D BP

Vitamin D BP (Vitamin D binding protein), also known as group-specific component and GC-globulin, is a 58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family. It was initially discovered as a major liver-derived polymorphic protein and was called group-specific component or GC in 1959 (1). Mature human Vitamin D BP is 458 amino acids (aa) in length. It contains three albumin-type domains that are accompanied by 14 intramolecular disulfide bonds (2). Mature human Vitamin D BP  is 77% aa identical to mouse Vitamin D BP. Vitamin D BP is able to bind to various forms of Vitamin D including vitamin D2, the 25‑hydroxylated forms and the active hormonal product, 1,25-dihydroxyvitamin D (Calcitriol). The major proportion of Vitamin D in blood is bound to Vitamin D BP, which transports vitamin D metabolites between skin, liver and kidney and then on to various target tissues (3). Vitamin D BP is implemented as a cause for multiple sclerosis (4). There is a strong suggestion that vitamin D and Vitamin D BP  gene polymorphism are involved in occurrence of type 2 diabetes mellitus (5). Vitamin D BP  has been associated with inflammatory process, stimulation of chemotaxis by phagocytic neutrophils and the activation and stimulation of phagocytic function by macrophages (6, 7).

References

  1. Hirschfeld, J. (1959) Acta. Pathol. Microbiol. Scand. 47:160.
  2. Schoentgen, F. et al. (1986) Biochim. Biophys. Acta. 871:189.
  3. Verboven, C. et al. (2002) Nat. Struct. Biol. 9:131.
  4. Langer-Gould, A. et al. (2018) Nutrients. 10:E184.
  5. Rahman, M.M. et al. (2017) Gene 636:42.
  6. Binder, R. et al. (1999) Mol. Immunol. 36:885.
  7. Kew, R.R. et al. (1995) J Immunol. 155:5369.

Long Name

Vitamin D Binding Protein

Alternate Names

DBP, DBP-MAF, Gc, Gc-MAF, VDBG, VDBP

Entrez Gene IDs

2638 (Human); 14473 (Mouse); 24384 (Rat)

Gene Symbol

GC

UniProt

P02774

Additional Vitamin D BP Products

Product Documents for Recombinant Human Vitamin D BP His-tag Protein, CF

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Product Specific Notices for Recombinant Human Vitamin D BP His-tag Protein, CF

For research use only

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