Recombinant Human VSTM2B Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10060

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

A6NLU5.2

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all VSTM2B Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VSTM2B protein
Human VSTM2B
(Ala29-Thr263)
Accession # A6NLU5.2		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala29

Predicted Molecular Mass

53 kDa

SDS-PAGE

60-72 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 1- 7.5 μg/mL

Measured by its binding ability in a functional ELISA.
When Goat Anti-Human IgG Fc Antibody  (Catalog # G-102-C) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human VSTM2B Fc Chimera Avi-tag (Catalog # AVI10060) that produces 50% of the optimal binding response is found to be approximately 1-10 ng/mL.

Scientific Data Images for Recombinant Human VSTM2B Fc Chimera Avi-tag Protein, CF

Recombinant Human VSTM2B Fc Chimera Avi-tag Protein Binding Activity

Recombinant Human VSTM2B Fc Chimera Avi-tag Protein Binding Activity

When Goat Anti-Human IgG Fc Antibody (G-102-C) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human VSTM2B Fc Chimera Avi-tag (Catalog # AVI10060) that produces 50% of the optimal binding response is found to be approximately 1-10 ng/mL.
Recombinant Human VSTM2B Fc Chimera Avi-tag Protein SDS-PAGE

Recombinant Human VSTM2B Fc Chimera Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human VSTM2B Fc Chimera Avi-tag (Catalog # AVI10060) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-72 kDa and 120-144 kDa.

Formulation, Preparation and Storage

AVI10060
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSTM2B

V-set and transmembrane domain-containing protein 2B (VSTM2B) is a type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. There have been 8 VSTM family members identified and several have been shown to exhibit immunosuppressive activity (1). Human VSTM2B consists of an extracellular domain (ECD) with an Ig-like V-type domain, a transmembrane domain, and single amino acid (aa) in the cytoplasm. The mature ECD of human VSTM2B shares 86% and 85% aa sequence identity with mouse and rat VSTM2B, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. Our in-house data show that VSTM2B inhibits the human T cell activation, including anti-CD3 induced IL-2, IFN-gamma secretion, and T cell proliferation. Our Avi-tag Biotinylated Recombinant VSTM2B features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Long Name

V-Set And Transmembrane Domain Containing 2B

Alternate Names

V-set and transmembrane domain-containing protein 2B

Entrez Gene IDs

342865 (Human); 58188 (Mouse); 361560 (Rat)

Gene Symbol

VSTM2B

UniProt

A6NLU5.2

Additional VSTM2B Products

Product Documents for Recombinant Human VSTM2B Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human VSTM2B Fc Chimera Avi-tag Protein, CF

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