Recombinant Human XIAP (Full Length) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 822-XF
Key Product Details
Product Specifications
Source
E. coli-derived human XIAP protein
| ATVID | 6-His tag | SSNG | Human XIAP (Met1-Ser497) Accession # AAC50373 |
| N-terminus | C-terminus | ||
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala
Predicted Molecular Mass
58 kDa
SDS-PAGE
50-60 kDa, reducing conditions
Activity
Measured by its ability to inhibit DEVD-AFC cleavage activity by Recombinant Human Caspase-7 (Catalog # 823-C7).
The IC50 for inhibition of rhCaspase-7 activity is <50 nM, as measured under the described conditions.
The IC50 for inhibition of rhCaspase-7 activity is <50 nM, as measured under the described conditions.
Formulation, Preparation and Storage
822-XF
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: XIAP
XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits Caspase-3 and Caspase-7. The BIR3 domain inhibits Caspase-9. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP‑BIR2 and BIR3 domains.
SUPPLEMENTARY METHODS
XIAP inhibits DEVD-AFC cleavage by activated cell extracts.
See http://www.RnDSystems.com/go/IAPProtocols">www.RnDSystems.com/go/IAPProtocols
XIAP inhibits DEVD-AFC cleavage by Recombinant Human Caspase-3.
Recombinant Human Caspase‑3 (Catalog # http://www.rndsystems.com/product_results.aspx?k=707-C3">707-C3) can be used in the Activity Assay Protocol in place of Casapase-7 by diluting to initial concentration of 0.1 ng/μL in Step 1.
The IC50 for inhibition of rhCaspase-3 activity is typically 10-200 nM.
References
- Deveraux, Q. et al. (1998) EMBO J. 17:2215.
- Deveraux, Q. et al. (1999) EMBO J. 18:5242.
- Verhagen, A. et al. (2000) Cell 102:43.
- Chai, J. et al. (2001) Cell 104:769.
- Riedl, S. (2001) Cell 104:791.
- Suzuki, Y. (2001) J. Biol. Chem. 276:27058.
- Ekert, P. et al. (2001) J. Cell Biol. 152:48.
- Du, C. et al. (2000) Cell 102:33.
Long Name
X-linked Inhibitor of Apoptosis
Alternate Names
BIRC4
Gene Symbol
XIAP
UniProt
Additional XIAP Products
Product Documents for Recombinant Human XIAP (Full Length) Protein, CF
Product Specific Notices for Recombinant Human XIAP (Full Length) Protein, CF
For research use only
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