Recombinant Mouse Alkaline Phosphatase/ALPL Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2910-AP
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Alkaline Phosphatase/ALPL protein
Phe18-Gly503, with a C-terminal 6-His tag
Phe18-Gly503, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Phe18
Predicted Molecular Mass
54 kDa
SDS-PAGE
77 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).
The specific activity is >40,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >40,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2910-AP
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Alkaline Phosphatase/ALPL
Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP) (1). The Alpl gene is a key regulator of bone mineralization in mice (2). A variety of mutations in the human ALPL gene leads to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 504 to 524) is not present in the mature form.
References
- Terao, M. and B. Mintz (1987) Proc. Natl. Acad. Sci. USA 84:7051.
- Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9445.
- Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Long Name
Alkaline Phosphatase Liver
Alternate Names
Akp2, AP-TNAP, HOPS, TNAP, TNSALP
Gene Symbol
ALPL
UniProt
Additional Alkaline Phosphatase/ALPL Products
Product Documents for Recombinant Mouse Alkaline Phosphatase/ALPL Protein, CF
Product Specific Notices for Recombinant Mouse Alkaline Phosphatase/ALPL Protein, CF
For research use only
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