Recombinant Mouse Aminopeptidase N/CD13 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2335-ZN

R&D Systems, part of Bio-Techne
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2335-ZN-010
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Key Product Details

Source

NS0

Accession #

P97449

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Aminopeptidase N/CD13 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Aminopeptidase N/CD13 protein
Lys69-Ser966, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys69

Predicted Molecular Mass

104 kDa

SDS-PAGE

111-123 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC).
The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

2335-ZN
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aminopeptidase N/CD13

The mouse ANPEP gene encodes Aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, Aminopeptidase M, CD13, or membrane protein p161 (1-3). The deduced amino acid sequence of mouse APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (4).

References

  1. Chen, H. et al. (1996) J. Immunol. 157:2593.
  2. Larsen, S.L. et al. (1996) J. Exp. Med. 184:183.
  3. Hansen, A.S. et al. (1993) Eur. J. Immunol. 23:2358.
  4. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 289 Academic Press, San Diego.

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Entrez Gene IDs

290 (Human); 16790 (Mouse); 397520 (Porcine); 102115663 (Cynomolgus Monkey)

Gene Symbol

ANPEP

UniProt

P97449

Additional Aminopeptidase N/CD13 Products

Product Documents for Recombinant Mouse Aminopeptidase N/CD13 Protein, CF

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Product Specific Notices for Recombinant Mouse Aminopeptidase N/CD13 Protein, CF

For research use only

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