Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2, CF

R&D Systems, part of Bio-Techne | Catalog # 9796-AN

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

AAD55358

Conjugate

Unconjugated

Applications

Bioactivity

View all Angiopoietin-like Protein 2/ANGPTL2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 2/ANGPTL2 protein
Mouse ANGPTL2
(Ala260-His493)
Accession # AAD55358		 HP GGGSGGGSGGGS HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala260

Predicted Molecular Mass

29 kDa

SDS-PAGE

28-39 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Angiopoietin‑like Protein 2/ANGPTL2 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) that produces 50% of the optimal binding response is 12-96 ng/mL.

Scientific Data Images for Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2, CF

Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2 Binding Activity

Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2 Binding Activity

When Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2 (Catalog # 9796-AN) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 12-96 ng/mL.

Formulation, Preparation and Storage

9796-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 2/ANGPTL2

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2 is a secreted 56 kDa glycoprotein that contains a N-terminal coiled coil domain and a C-terminal fibrinogen like domain (1). ANGPTL2 is one of the seven members of the Angiopoietin Like family proteins, which are structurally similar to angiopoietins (2). Within mature mouse ANGPTL2, amino acid 260-493 contains the C-terminal fibrinogen like domain, and this domain shares 99% and 100% sequence identity with human and rat homologs, respectively. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6-7). ANGPTL2 also function as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled coil domain (8). Receptor of ANGPTL2 is recently identified as LILRB2, and both the coiled coil domain and fibrinogen like domain are required for optimal binding (9).

References

  1. Kim, I. et al. (1999) J Biol Chem 274:26523.
  2. Santulli, G. et al. (2014) Front Endocrinol 5:4.
  3. Tabata, M. et al. (2009) Cell Metab 10:178.
  4. Kubota, Y. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:13502.
  5. Kikuchi, R. et al. (2008) Cancer Res 68:5067.
  6. Odagiri, H. et al. (2014) Sci Signal 7:ra7.
  7. Endo, M. et al. (2012) Cancer Res 72:1784.
  8. Broxmeyer, H.E. et al. (2012) Blood Cells Mol Dis 48:25.
  9. Deng, M. et al. (2014) blood 124:924.

Alternate Names

ANGPTL2, ANGRP2

Entrez Gene IDs

23452 (Human); 26360 (Mouse)

Gene Symbol

ANGPTL2

UniProt

AAD55358

Additional Angiopoietin-like Protein 2/ANGPTL2 Products

Product Documents for Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2, CF

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Product Specific Notices for Recombinant Mouse Angiopoietin-like Protein 2/ANGPTL2, CF

For research use only

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