Recombinant Mouse ASAH2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3558-AH
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse ASAH2/N-acylsphingosine Amidohydrolase-2 protein
Thr34-Thr756, with an N-terminal 6-His tag
Accession # NP_061300
Thr34-Thr756, with an N-terminal 6-His tag
Accession # NP_061300
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
81 kDa
SDS-PAGE
113 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid.
The specific activity is > 3,000 pmol/min/µg, as measured under the described conditions.
The specific activity is > 3,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
3558-AH
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl and Sodium Cholate. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ASAH2/N-acylsphingosine Amidohydrolase-2
The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, also known as neutral ceramidase. Neutral ceramidase is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). A major physiological function of neutral ceramidase is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).
References
- Tani, M. et al. (2003) J. Biol. Chem. 278:10523.
- Kono, M. et al. (2006) J. Biol. Chem. 281:7324.
- Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.
Long Name
Neutral Ceramidase
Alternate Names
Nacylsphingosine Amidohydrolase2
Gene Symbol
ASAH2
UniProt
Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products
Product Documents for Recombinant Mouse ASAH2 Protein, CF
Product Specific Notices for Recombinant Mouse ASAH2 Protein, CF
For research use only
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