Recombinant Mouse beta-Defensin 3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5987-BD

R&D Systems, part of Bio-Techne
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5987-BD-050
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Key Product Details

Source

E. coli

Accession #

Q9WTL0

Conjugate

Unconjugated

Applications

Bioactivity

View all beta-Defensin 3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse beta-Defensin 3 protein
Lys23-Lys63

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys23

Predicted Molecular Mass

4.6 kDa

SDS-PAGE

5 kDa, reducing conditions

Activity

Measured by its anti-microbial activity against E. coli. Ganz, T. et al. (2003) Nat. Rev. Immunol. 3:710.
The ED50 for this effect is 4-20 μg/mL.

Formulation, Preparation and Storage

5987-BD
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution
Reconstitute at 500 μg/mL in 4 mM HCl.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Defensin 3

beta-defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta-defensins which are distinguished from alpha-defensins by the connectivity pattern of their three intramolecular disulfide bonds (1). The 41 aa mature mouse BD3 shares 38% and 59% aa sequence identity with human and rat BD3, respectively (2, 3). It shares 14% - 46% aa sequence identity with other mouse beta-defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is up-regulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds (2, 4 ‑ 6). BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression (7). In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S (8). BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast (2, 9). BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability (4, 10). Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties (11, 12). BD3 inhibits viral infectivity by interacting directly with HIV-1 and its coreceptor CXCR4 (5, 13) and with HSV glycoprotein B and its receptor heparan sulfate (14), and by forming a protective coating on the surface of influenza virus target cells (15).

References

  1. Dhople, V. et al. (2006) Biochim. Biophys. Acta. 1758:1499.
  2. Bals, R. et al. (1999) Infect. Immun. 67:3542.
  3. Schibli, D.J. et al. (2002) J. Biol. Chem. 277:8279.
  4. Garcia, J.-R.C. et al. (2001) Cell Tissue Res. 306:257.
  5. Quinones-Mateu, M.E. et al. (2003) AIDS 17:F39.
  6. Sorensen, O.E. et al. (2006) J. Clin. Invest. 116:1878.
  7. Varoga, D. et al. (2005) Arthritis Rheum. 52:1736.
  8. Taggart, C.C. et al. (2003) J. Immunol. 171:931.
  9. Joly, S. et al. (2004) J. Clin. Microbiol. 42:1024.
  10. Chen, X. et al. (2007) Eur. J. Immunol. 37:434.
  11. Kluver, E. et al. (2005) Biochemistry 44:9804.
  12. Wu, Z. et al. (2003) Proc. Natl. Acad. Sci. 100:8880.
  13. Feng, Z. et al. (2006) J. Immunol. 177:782.
  14. Hazrati, E. et al. (2006) J. Immunol. 177:8658.
  15. Leikina, E. et al. (2005) Nat. Immunol. 6:995.

Alternate Names

BD14, BD3, betaDefensin 3, DEFB103A, DEFB103B, DEFB3, HBD-3

Entrez Gene IDs

55894 (Human)

Gene Symbol

DEFB103B

UniProt

Q9WTL0

Additional beta-Defensin 3 Products

Product Documents for Recombinant Mouse beta-Defensin 3 Protein, CF

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Product Specific Notices for Recombinant Mouse beta-Defensin 3 Protein, CF

For research use only

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