Recombinant Mouse BST-2/Tetherin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9940-BS

R&D Systems, part of Bio-Techne
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9940-BS-050
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Key Product Details

Source

E. coli

Accession #

Q8R2Q8

Conjugate

Unconjugated

Applications

Bioactivity

View all BST-2/Tetherin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse BST-2/Tetherin protein
Thr52-Ser152, with a C-Terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr52

Predicted Molecular Mass

12 kDa

SDS-PAGE

13 kDa and 28 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse BST-2/Tetherin (Catalog # 9940-BS) is immobilized at 1 µg/mL (100 µL/well), Recombinant Viral Dengue Virus 4 Envelope Fc Chimera binds with an ED50 of 0.2-1.6 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 9940-BS in the following applications:

Scientific Data Images for Recombinant Mouse BST-2/Tetherin Protein, CF

Recombinant Mouse BST-2/Tetherin Protein Binding Activity

Recombinant Mouse BST-2/Tetherin Protein Binding Activity

When Recombinant Mouse BST-2/Tetherin (Catalog # 9940-BS) is immobilized at 1 µg/mL (100 µL/well), Recombinant Viral Dengue Virus 4 Envelope Fc Chimera binds with an ED50 of 0.2-1.6 μg/mL.
Recombinant Mouse BST-2/Tetherin Protein SDS-PAGE

Recombinant Mouse BST-2/Tetherin Protein SDS-PAGE

2 μg/lane of Recombinant Mouse BST-2/Tetherin (Catalog # 9940-BS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 13 kDa and 28 kDa.

Formulation, Preparation and Storage

9940-BS
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BST-2/Tetherin

BST-2, also known as tetherin or CD317, is a type II transmembrane protein that is constitutively expressed in several cell types including T cells, B cells, monocytes and macrophages, as well as on several cancer cell lines including the B cell lineage in multiple myeloma (1, 2). Originally named bone marrow stromal cell antigen 2, BST-2 was identified to inhibit viral replication due to its unusual topology (3-5). The extracellular C-terminus of the protein is modified with GPI (glycosylphosphatidylinositol) membrane anchor, which enables BST-2 to interact at each of its ends with the cell or the viral membrane. The ectodomain of the protein also contains three cysteine residues that can form intermolecular disulfide bonds to form parallel dimers (2). Mouse BST-2 consists of a 30 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 101 aa extracellular domain (ECD). Within the ECD, mouse BST-2 shares 41% and 71% aa sequence identity with human and rat BST-2, respectively. BST-2 was identified as a major mediator of the innate immune defense against enveloped viruses. BST-2 was shown to interact with formed viral particles tethering them to surface of infected cells, thereby reducing viral release (6). BST-2 inhibits the release of diverse group of enveloped viruses including members of the retrovirus, togavirus, filovirus, arenavirus, flavivirus, rhabdovirus, orthohepadnavirus, orthomyxovirus, poxvirus, paramyxovirus, and herpesvirus families (7-9). The mechanism BST-2 inhibition of viral budding was suggested to be through directly bridging the host and viral membranes through simultaneous embedding of its two opposing membrane anchors (9). To counteract BST-2 functions, many viruses have evolved to develop strategies to antagonize BST-2 function (9, 10). In addition to inhibiting viral release, BST-2 can act as an innate immune sensor of viral infections by acting as a pattern recognition receptor that activates NF-kappa B to induce inflammatory responses through interactions with the immunoglobulin-like transcript 7 (ILT7/LILRA4) (10, 11).

References

  1. Evans, D.T. et al. (2010) Trens Microbiol. 18:388.
  2. Ohtomo, T. et al. (1999) Biochem. Biophys. Res. Commun. 258:583.
  3. Ishikawa, J. et al. (1995) Genomics. 26:527.
  4. Neil, S.J. et al. (2008) Nature. 451:425.
  5. Kupzig, S. et al. (2003) Traffic. 4:694.
  6. Janvier K. et al. (2012) Curr HIV Res. 10:315.
  7. Evans D.T. et al. (2010) Trends Microbiol. 18:388.
  8. Mahauad-Fernandez W.D. et al. (2012) Immun Inflamm Dis. 4:4.
  9. Swiecki M. et al. (2013) Mol Immunol. 54:132.
  10. Sauter D. (2014) Front Microbiol. 5:163.
  11. Hotter D. et al. (2013) J Mol Biol. 425:4956.

Long Name

Bone Marrow Stromal Cell Antigen 2

Alternate Names

BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin

Entrez Gene IDs

684 (Human); 69550 (Mouse)

Gene Symbol

BST2

UniProt

Q8R2Q8

Additional BST-2/Tetherin Products

Product Documents for Recombinant Mouse BST-2/Tetherin Protein, CF

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Product Specific Notices for Recombinant Mouse BST-2/Tetherin Protein, CF

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