Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8997-BT

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

A4QPC6

Conjugate

Unconjugated

Applications

Bioactivity

View all BTN2A2/Butyrophilin 2A2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BTN2A2/Butyrophilin 2A2 protein
Gln30-Trp248, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln30 inferred from enzymatic pyroglutamate treatment revealing Phe31

Predicted Molecular Mass

26 kDa

SDS-PAGE

38-45 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 1-5 μg/mL.

Scientific Data Images for Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF

Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein Bioactivity

Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein Bioactivity

Recombinant Mouse BTN2A2/Butyrophilin 2A2 inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 ug/mL.

Formulation, Preparation and Storage

8997-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTN2A2/Butyrophilin 2A2

Butyrophilin 2A2 (BTN2A2) is a widely expressed 65 kDa type I transmembrane glycoprotein that functions as a negative regulator of immune responses (1). Mature mouse Butyrophilin 2A2 consisits of a 215 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 249 aa cytoplasmic domain. Within the Ig-like domains, mouse Butyrophilin 2A2 shares 71% and 90% aa sequence identity with human and rat Butyrophilin 2A2, respectively. Alternative splicing generates an additional isoform of mouse Butyrophilin 2A2 with a deletion and substitution in the cytoplasmic region. Within the immune system, Butyrophilin 2A2 is expressed on thymic epithelial cells, naïve B cells, splenic NK cells, dendritic cells, and peritoneal macrophages and is up-regulated with cell activation (2, 3). Butyrophilin 2A2 inhibits T cell proliferation and activation and enhances the development of FoxP3+ regulatory T cells (2, 3). Its up-regulation in the hippocampus is associated with schizophrenia (4).

References

  1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
  2. Smith, I.A. et al. (2010) J. Immunol. 184:3514.
  3. Ammann, J.U. et al. (2013) J. Immunol. 190:5030.
  4. Sinkus, M.L. et al. (2013) Brain Behav. Immun. 32:51.

Long Name

Butyrophilin Subfamily 2 Member A2

Alternate Names

BTF2, BTN2, Butyrophilin 2

Entrez Gene IDs

10385 (Human); 238555 (Mouse); 306957 (Rat)

Gene Symbol

BTN2A2

UniProt

A4QPC6

Additional BTN2A2/Butyrophilin 2A2 Products

Product Documents for Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF

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Product Specific Notices for Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF

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