Recombinant Mouse Cadherin-6/KCAD Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6826-CA
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Cadherin-6/KCAD protein
Met1-Ala615 with a C-terminal 6-His tag
Met1-Ala615 with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asn22 (pro-protein) & Ser54 (mature protein)
Predicted Molecular Mass
67.0 kDa (pro-protein) & 63.3 kDa (mature protein)
SDS-PAGE
90-105 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of Caki-2 human clear cell carcinoma epithelial cells.
The ED50 for this effect is 0.2-1.0 μg/mL after a 30 minutes incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
The ED50 for this effect is 0.2-1.0 μg/mL after a 30 minutes incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
6826-CA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in DPBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cadherin-6/KCAD
calcium‑dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Mouse Cadherin‑6 is synthesized with a 18 amino acid (aa) signal peptide and a 35 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 562 amino acid (aa) extracellular domain (ECD) that contains five tandem Cadherin repeats, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (3). Within the ECD, mouse Cadherin‑6 shares 96% aa sequence identity with human and rat Cadherin‑10. It interacts homotypically as well as heterotypically with Cadherin‑9 and more weakly with Cadherins‑7, ‑10, and ‑14 (4, 5). Cadherin‑6 is expressed primarily in the kidney and brain, both during development and in the adult (6‑9). It is enriched in renal proximal tubule epithelial cells and their precursors at sites of cell‑cell contact as well as on basolateral membranes (7, 10, 11). In the nervous system, Cadherin‑6 is neuronally expressed in discrete regions of the neural plate, neural crest cells that give rise to peripheral nerves, and the brain (3, 6). Cadherin‑6 is additionally expressed in the developing retina, osteoclasts and their progenitors, ovarian epithelium, and endometrium during the follicular phase of the menstrual cycle (12‑15). It is also expressed in carcinomas of the kidney, ovary, and liver (8, 10, 11, 14).
References
- Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
- Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
- Inoue, T. et al. (1997) Dev. Biol. 183:183.
- Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
- Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.
- Inoue, T. et al. (1998) Dev. Dyn. 211:338.
- Xiang Y.-Y. et al. (1994) Cancer Res. 54:3034.
- Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
- Cho, E.A. et al. (1998) Development 125:803.
- Paul, R. et al. (1997) Cancer Res. 57:2741.
- Shimazui, T. et al. (2000) Eur. Urol. 38:331.
- Ruan, G. et al. (2006) Mech. Dev. 123:881.
- Mbalaviele, G. et al. (1998) J. Cell Biol. 141:1467.
- Sellar, G.C. et al. (2001) Cancer Res. 61:6977.
- Getsios, S. et al. (1998) Dev. Dyn. 211:238.
Alternate Names
Cadherin6, CDH6, K-Cadherin
Entrez Gene IDs
1004 (Human)
Gene Symbol
CDH6
UniProt
Additional Cadherin-6/KCAD Products
Product Documents for Recombinant Mouse Cadherin-6/KCAD Protein, CF
Product Specific Notices for Recombinant Mouse Cadherin-6/KCAD Protein, CF
For research use only
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