Recombinant Mouse Carbonic Anhydrase XII Protein, CF

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Mouse CA12 is predicted to be a type I membrane enzyme (2, 3). The secreted, purified recombinant mouse CA12 corresponds to the extracellular domain and has both CA activity and esterase activity. To our knowledge, this is the first description of purified recombinant mouse CA12 with enzymatic activity.