Recombinant Mouse CCL20/MIP-3 alpha Protein
R&D Systems, part of Bio-Techne | Catalog # 760-M3
Key Product Details
Product Specifications
Source
E. coli-derived mouse CCL20/MIP-3 alpha protein
Ala27-Met96
Ala27-Met96
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala27
Predicted Molecular Mass
7.9 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR6.
The ED50 for the chemotactic effect is 2-6 ng/mL.
The ED50 for the chemotactic effect is 2-6 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 760-M3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 760-M3/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCL20/MIP-3 alpha
MIP-3 alpha, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20 - 28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71 and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively.
MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.
References
- Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
- Hromas, R. et al. (1997) Blood 89:3315.
- Greaves, D. R. et al. (1997) J. Exp. Med. 186: 857.
- Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.
Alternate Names
exodus-1, LARC, MIP-3 alpha, MIP3 alpha
Gene Symbol
CCL20
UniProt
Additional CCL20/MIP-3 alpha Products
Product Documents for Recombinant Mouse CCL20/MIP-3 alpha Protein
Product Specific Notices for Recombinant Mouse CCL20/MIP-3 alpha Protein
For research use only
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