Recombinant Mouse CCL21/6Ckine Protein

R&D Systems, part of Bio-Techne | Catalog # 457-6C

R&D Systems, part of Bio-Techne
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Carrier Free
457-6C-025/CF
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With Carrier
457-6C-025
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Key Product Details

Source

E. coli

Accession #

P84444.1

Conjugate

Unconjugated

Applications

Bioactivity

View all CCL21/6Ckine Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse CCL21/6Ckine protein
Ser24-Gly133

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser24

Predicted Molecular Mass

12 kDa

SDS-PAGE

16-17 kDa, reducing conditions

Activity

Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL).
The ED50 for this effect is 50-200 ng/mL.

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR7.
The ED50 for this effect is 3-15 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 457-6C
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 457-6C/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL21/6Ckine

CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a 12 kDa homeostatic chemokine that plays an important role in adaptive immune responses and inflammation (1). Unlike other CC chemokines, mouse CCL21 has a 36 amino acid (aa) C-terminal extension which mediates its attachment to carbohydrate structures and extracellular matrix components (2, 3). Mature mouse CCL21 shares 71% and 84% aa sequence identity with human and rat CCL21, respectively (4‑6). In mouse, three genes encode CCL21, with B and C forms being identical and the A form carrying serine at aa 65 instead of leucine. Both human and mouse CCL21 signal through the chemokine receptor CCR7, while mouse CCL21 additionally can signal through CXCR3 (7). CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) (8‑10). Immobilized CCL21 promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation (8, 9). DC interaction with the anchored chemokine can induce CCL21 cleavage and release of an 8 kDa fragment that lacks the C‑terminal extension (10). During chronic inflammation or tissue damage, CCL21 is up‑regulated on local vascular endothelial cells, macrophages, T cells, and neurons (11‑14). In these settings, it promotes fibrosis, inflammatory cytokine production, and neuropathic pain (12‑14). The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients (11, 13). CCL21 has been shown to exert either angiogenic or angiostatic effects (11, 15, 16). These effects, in combination with the ability of CCL21 to attract immune suppressor cells (Treg and MDSC) to a tumor site can have positive or negative effects on tumor progression (16, 17).

References

  1. Forster, R. et al. (2008) Nat. Rev. Immunol. 8:362.
  2. Yang, B.G. et al. (2007) J. Immunol. 179:4376.
  3. Rey-Gallardo, A. et al. (2010) Glycobiology 20:1139.
  4. Tanabe, S. et al. (1997) J. Immunol. 159:5671.
  5. Hromas, R. et al. (1997) J. Immunol. 159:2554.
  6. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
  7. Jenh, C. et al. (1999) J. Immunol. 162:3765.
  8. Tal, O. et al. (2011) J. Exp. Med. 208:2141.
  9. Friedman, R.S. et al. (2006) Nat. Immunol. 7:1101.
  10. Schumann, K. et al. (2010) Immunity 32:703.
  11. Pickens, S.R. et al. (2012) Arthritis Rheum. 64:2471.
  12. Sakai, N. et al. (2006) Proc. Natl. Acad. Sci. USA 103:14098.
  13. Damas, J.K. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:614.
  14. Biber, K. et al. (2011) EMBO J. 30:1864.
  15. Soto, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8205.
  16. Vicari, A.P. et al. (2000) J. Immunol. 165:1992.
  17. Shields, J.D. et al. (2010) Science 328:749.

Alternate Names

6Ckine, exodus-2, SCYA21, SLC, TCA-4

Entrez Gene IDs

6366 (Human); 18829 (Mouse); 298006 (Rat)

Gene Symbol

CCL21

UniProt

P84444.1

Additional CCL21/6Ckine Products

Product Documents for Recombinant Mouse CCL21/6Ckine Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 457-6C (with carrier)
Download SDS 457-6C/CF (carrier free)

Product Specific Notices for Recombinant Mouse CCL21/6Ckine Protein

For research use only

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