Recombinant Mouse CD155/PVR Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9670-CD

R&D Systems, part of Bio-Techne
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9670-CD-050
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Key Product Details

Source

NS0

Accession #

NP_081790

Structure / Form

DIsulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD155/PVR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD155/PVR protein
Mouse CD155/PVR
(Asp29-Leu348)
Accession # NP_081790		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp29

Predicted Molecular Mass

62 kDa

SDS-PAGE

92-108 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse CD96 (Catalog # 5690-CD) is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD155/PVR Fc Chimera that produces 50% optimal binding response is 6-36 ng/mL.

Scientific Data Images for Recombinant Mouse CD155/PVR Fc Chimera Protein, CF

Recombinant Mouse CD155/PVR Fc Chimera Protein Bioactivity

Recombinant Mouse CD155/PVR Fc Chimera Protein Bioactivity

When Recombinant Mouse CD96 (Catalog # 5690-CD) is coated at 1 µg/mL, 100 µL/well, Recombinant Mouse CD155/PVR Fc Chimera (Catalog # 9670-CD) binds with an ED50 of 6-36 ng/mL.

Formulation, Preparation and Storage

9670-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155, which may play a role in cancer cell invasion and migration, binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically (3, 4). Mature mouse CD155 consists of a 318 amino acid (aa) extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa transmembrane segment, and a 38 aa cytoplasmic tail. Within the ECD, mouse CD155 shares 46%, 73%, and 44% aa sequence identity with human CD155, rat CD155, and mouse Nectin-2, respectively. The V-type domain of CD155 mediates all binding, including to polio virus (1), and alternative splicing within this domain in humans can modulate ligand binding (5). CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 6-9). It is preferentially expressed on Th17 cells compared to Th2 cells (10), and its activation promotes the development of Th1 responses (11). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 7, 12). Binding of monocyte DNAM-1 to endothelial cell CD155 promotes transendothelial migration (8). The expression of CD155 on mouse CD8+ thymocytes prevents their premature exit from the thymus (13). Within intestinal Peyer's patches, follicular dendritic cell CD155 activates follicular helper T cells via DNAM-1 or CD96 binding (7-9, 14). CD155 also binds the inhibitory ligand TIGIT on NK and some mature T cells, antagonizing DNAM-1 effects (7, 14, 15).

References

  1. Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197.
  2. Ravens, I. et al. (2003) Biochem. Biophys. Res. Commun. 312:1364.
  3. Sloan, K. et al. (2004) BMC Cancer. 4: 73.
  4. Sato, T. et al. (2004) Genes to Cells 9:791.
  5. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
  6. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177.
  7. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
  8. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  9. Maier, M.K. et al. (2007) Eur. J. Immunol. 37 :2214.
  10. Lozano, E. et al. (2013) J. Immunol. 191:3673.
  11. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644.
  12. Chan, C.J. et al. (2010) J. Immunol. 184:902.
  13. Qui, Q. et al. (2010) J. Immunol. 184:1681.
  14. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
  15. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.

Long Name

Poliovirus Receptor

Alternate Names

CD155, HVED, Necl-5, PVR, PVS

Entrez Gene IDs

5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)

Gene Symbol

PVR

UniProt

NP_081790

Additional CD155/PVR Products

Product Documents for Recombinant Mouse CD155/PVR Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse CD155/PVR Fc Chimera Protein, CF

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