Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 852-CD
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse CD30/TNFRSF8 protein
Met | Mouse CD30 (Phe19 - Thr281) Accession # Q60846 |
IEGRDMD | Human IgG1 (Pro100 - Lys330) |
6-His tag |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
55 kDa (monomer)
SDS-PAGE
90-100 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When rmCD30 Ligand (Catalog # 732-CL) is immobilized at 200 ng/well, the concentration of rmCD30 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.5-2.5 ng/mL
When rmCD30 Ligand (Catalog # 732-CL) is immobilized at 200 ng/well, the concentration of rmCD30 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.5-2.5 ng/mL
Formulation, Preparation and Storage
852-CD
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD30/TNFRSF8
References
- Kennedy, M.K. et al. (2006) Immunology 118:143.
- Tarkowski, M. (2003) Curr. Opin. Hematol. 10:267.
- Bowen, M.A. et al. (1996) J. Immunol. 156:442.
- Hamann, D. et al. (1996) J. Immunol. 156:1387.
- Shanebeck, S.D. et al. (1995) Eur. J. Immunol. 25:2147.
- Gruss, H.-J. et al. (1994) Blood 83:2045.
- Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174.
- Del Prete, G. et al. (1995) J. Exp. Med. 182:1655.
- Harlin, H. et al. (2002) J. Immunol. 169:2451.
- Amakawa, R. et al. (1996) Cell 84:551.
- Chiarle, R. et al. (1999) J. Immunol. 163:194.
- Vinante, F. et al. (2002) Blood 99:52.
- Hansen, H.P. et al. (1995) Int. J. Cancer 63:750.
- Hansen, H.P. et al. (2000) J. Immunol. 165:6703.
- Hargreaves, P.G. and A. Al-Shamkhani (2002) Eur. J. Immunol. 32:163.
Alternate Names
CD30, TNFRSF8
Gene Symbol
TNFRSF8
UniProt
Additional CD30/TNFRSF8 Products
Product Documents for Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein, CF
For research use only
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