Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 7190-CD
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse CD320/TCblR/8D6A protein
| Mouse CD320 (Met1 - Gly208) Accession # Q9Z1P5 |
IEGRMDP | Mouse IgG2A (Glu98 - Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala29
Predicted Molecular Mass
46.7 kDa (monomer)
SDS-PAGE
70-90 kDa, reducing conditions
Activity
Measured by its ability to bind plasma Cobalamin (Cbl) bound Transcobalamin (TC). Quadros, E.V. et al. (2009) Blood 113:186.
When Recombinant Mouse CD320/TCblR/8D6A Fc Chimera is coated at 1 μg/mL (100 μL/well), it can bind human plasma TC (dilutions from 1:6 to 1:4000) in the presence of Cbl.
When Recombinant Mouse CD320/TCblR/8D6A Fc Chimera is coated at 1 μg/mL (100 μL/well), it can bind human plasma TC (dilutions from 1:6 to 1:4000) in the presence of Cbl.
Formulation, Preparation and Storage
7190-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 500 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD320/TCblR/8D6A
CD320, also known as the Transcobalamin II receptor (TCblR) and 8D6A, is an approximately 60 kDa transmembrane member of the LDL receptor family (1). Mature mouse CD320 consists of a 180 amino acid (aa) extracellular domain with two cysteine-rich LDLR-A repeats, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2, 3). Within the extracellular domain, mouse CD320 shares 52% aa sequence identity with human and rat CD320. Alternate splicing of mouse CD320 generates an additional isoform with a substitution of the N-terminal 40 aa. CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer in the kidney, placenta, liver, intestine, and secondary lymphoid organs (3 - 5). It functions as an endocytic receptor for circulating complexes of Cobalamin (Vitamin B12) and Transcobalamin II (3). In the immune system, CD320 is expressed by germinal center B cells and follicular dendritic cells (FDC) and provides a costimulatory signal to B cells during the germinal center reaction (2, 6 - 8). CD320 also cooperates with CD44 in promoting the development of B cell lymphomas (9).
References
- May, P. et al. (2007) Ann. Med. 39:219.
- Li, L. et al. (2000) J. Exp. Med. 191:1077.
- Quadros, E.V. et al. (2009) Blood 113:186.
- Bose, S. et al. (1995) J. Biol. Chem. 270:8152.
- Park, H.J. et al. (2009) Immune Netw. 9:138.
- Cho, W. et al. (2008) BMC Rep. 41:863.
- Zhang, X. et al. (2001) J. Immunol. 167:49.
- Park, C.-S. and Y.S. Choi (2005) Immunology 114:2.
- Li, L. et al. (2004) Blood 104:815.
Long Name
Transcobalamin II Receptor
Alternate Names
8D6A, CD320, TCblR
Gene Symbol
CD320
UniProt
Additional CD320/TCblR/8D6A Products
Product Documents for Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF
For research use only
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