Recombinant Mouse CD38 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4947-AC
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse CD38 protein
Leu45-Thr304, with a C-terminal 6-His tag
Leu45-Thr304, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu45
Predicted Molecular Mass
31 kDa
SDS-PAGE
36-43 kDa, reducing conditions
Activity
Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260.
The specific activity is >50,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >50,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4947-AC
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD38
CD38, also known as ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability; cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells (4, 5). CD38 has been shown to regulate oxytocin secretion, and may be involved in the development of complex social behaviors in mammals (6).
References
- Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
- Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
- Sun, L. et al. (1999) J. Cell Biol. 146:1161.
- Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
- Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
- Jin, D. et al. (2007) Nature 446:41.
Long Name
Cluster of Differentiation 38
Alternate Names
ADP-ribosyl Cyclase, CD38, Cyclic ADP-ribose Hydrolase
Gene Symbol
CD38
UniProt
Additional CD38 Products
Product Documents for Recombinant Mouse CD38 Protein, CF
Product Specific Notices for Recombinant Mouse CD38 Protein, CF
For research use only
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