Recombinant Mouse CD45 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 114-CD
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse CD45 protein
Gln24-Lys564 with a C-terminal 6-His tag
Gln24-Lys564 with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln24 predicted
Predicted Molecular Mass
59.5 kDa
SDS-PAGE
140-160 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When 0.5 μg/mL of rmCD45 is captured onto a Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050) coated plate, the concentration of rmGalectin-1 that produces 50% of the optimal binding is found to be approximately
0.45-1.8 μg/mL.
When 0.5 μg/mL of rmCD45 is captured onto a Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050) coated plate, the concentration of rmGalectin-1 that produces 50% of the optimal binding is found to be approximately
0.45-1.8 μg/mL.
Reviewed Applications
Read 3 reviews rated 5 using 114-CD in the following applications:
Formulation, Preparation and Storage
114-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD45
aa 30-169. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6-8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8-10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11-14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).
References
- Anderson, J.N. et al. (2004) FASEB J. 18:8.
- Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
- Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
- Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
- Ralph, S.J. et al. (1987) EMBO J. 6:1251.
- Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
- Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
- McNiell, L. et al. (2007) Immunity 27:425.
- Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
- Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
- Zhu, J.W. et al. (2008) Immunity 28:183.
- Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
- Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
- Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name
Cluster of Differentiation 45
Alternate Names
CD45, LCA, PTPRC, T200 Glycoprotein
Gene Symbol
PTPRC
UniProt
Additional CD45 Products
Product Documents for Recombinant Mouse CD45 Protein, CF
Product Specific Notices for Recombinant Mouse CD45 Protein, CF
For research use only
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