Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7335-CD

R&D Systems, part of Bio-Techne
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7335-CD-050
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Key Product Details

Source

NS0

Accession #

P01831

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD90/Thy1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD90/Thy1 protein
Mouse CD90/Thy1
(Gln20-Lys130)
Accession # P01831		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained, Gln20 predicted. Protein identity confirmed by MS analysis of tryptic fragments.

Predicted Molecular Mass

40 kDa (monomer)

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of human neutrophils.
The ED50 for this effect is 0.5-3 μg/mL.

Reviewed Applications

Read 7 reviews rated 5 using 7335-CD in the following applications:

Formulation, Preparation and Storage

7335-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD90/Thy1

CD90, also known as Thy1, is an approximately 25-35 kDa glycoprotein that mediates cellular adhesion and exerts wide ranging effects in different tissues (1, 2). Mature mouse CD90 contains one immunoglobulin-like domain followed by a GPI anchor (3-5). CD90 is expressed on hematopoietic progenitor cells, neurons, and activated vascular endothelial cells (6-8). Its species-specific expression in lymphoid cells allows its use as a thymocyte and pan T cell marker in mouse but not in human (1). CD90 can form dimers and higher order multimers (9). It binds to heparin, the proteoglycan Syndecan-4, and Integrins alphaM beta2, alphaV beta3, and  alphaV beta5 (8, 10-13). Through these interactions, CD90 inhibits neurite outgrowth (7), promotes astrocyte adhesion (10), mediates the adhesion and extravasation of leukocytes and melanoma cells (8, 12), supports the Thrombospondin-1 induced disassembly of fibroblast focal adhesions (14), and inhibits the activation of latent TGF-beta 1, myofibroblast differentiation, and lung fibrosis (13)

References

  1. Haeryfar, S.M.M. and D.W. Hoskin (2004) J. Immunol. 173:3581.
  2. Rege, T.A. and J.S. Hagood (2006) Biochim. Biophys. Acta 1763:991.
  3. Chang, H.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82:3819.
  4. Giguere, V. et al. (1985) EMBO J. 4:2017.
  5. Thomas, P.M. and L.E. Samelson (1992) J. Biol. Chem. 267:12317.
  6. Craig, W. et al. (1993) J. Exp. Med. 177:1331.
  7. Tiveron, M-C. et al. (1992) Nature 355:745.
  8. Wetzel, A. et al. (2004) J. Immunol. 172:3850.
  9. Mahanthappa, N.K. and P.H. Patterson (1992) Dev. Biol. 150:60.
  10. Avalos, A.M. et al. (2009) J. Cell Sci. 122:3462.
  11. Hueber, A.O. et al. (1992) J. Immunol. 148:3692.
  12. Saalbach, A. et al. (2005) Oncogene 24:4710.
  13. Zhou, Y. et al. (2010) J. Biol. Chem. 285:22382.
  14. Barker, T.H. et al. (2004) J. Biol. Chem. 279:23510.

Alternate Names

CD90, Thy1

Entrez Gene IDs

7070 (Human); 21838 (Mouse)

Gene Symbol

THY1

UniProt

P01831

Additional CD90/Thy1 Products

Product Documents for Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD90/Thy1 Fc Chimera Protein, CF

For research use only

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