Recombinant Mouse CLF-1/CLC Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10088-CL

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R&D Systems, part of Bio-Techne
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10088-CL-050
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Key Product Details

Source

CHO

Accession #

Q9JM58 (CLF-1), Q9QZM3 (CLC)

Conjugate

Unconjugated

Applications

Bioactivity

View all CLF-1/CLC Complex Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CLF-1/CLC Complex protein
Mouse CLF-1
(Gly40-Gly425)
Accession # Q9JM58		 SGGGGSGGGGSGGGGS
 Mouse CLC
(Leu28-Phe225)
Accession # Q9QZM3		 HHHHHH
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly40 & Ala41

Predicted Molecular Mass

67 kDa

SDS-PAGE

77-87 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells transfected with human CNTF R alpha.
The ED50 for this effect is 15-90 ng/mL.

Scientific Data Images for Recombinant Mouse CLF-1/CLC Complex Protein, CF

Recombinant Mouse CLF-1/CLC Complex Protein SDS-PAGE

Recombinant Mouse CLF-1/CLC Complex Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CLF‑1/CLC Complex (Catalog # 10088-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-87 kDa and 160-180 kDa, respectively.

Formulation, Preparation and Storage

10088-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLF-1/CLC Complex

Cardiotrophin-like cytokine (CLC), also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3), is a member of the IL-6 family of cytokines (1, 2). CLC associates with the secreted soluble cytokine-like factor 1 (CLF-1), a member of the cytokine type I receptor family, to form the heteromeric composite cytokine CLF-1/CLC (3, 5). CLC can also form an alternate composite cytokine with soluble ciliary neurotrophic factor receptor alpha (CNTF R alpha ) (4). Co-expression of CLC with either CLF-1 or CNTF R alpha is required for the formation of the composite cytokines and for CLC secretion (3-5). CLF-1/CLC binds to the membrane-associated CNTF R alpha to initiate the heterodimerization between gp130 and leukemia inhibitory factor receptor (LIFR) and stimulate the PI 3-kinase and the MAP kinase activity. The CLF-1/CLC complex displays activities only on those cells expressing the functional tripartite receptor complex (5). Mouse CLF-1 is a 425 amino acid (aa) protein that contains a 33 aa signal sequence and a 392 aa chain. Mouse CLC is a 225 amino acid (aa) protein that contains a 27 aa signal sequence and a 198 aa chain. Human and mouse CLF-1 share 94% amino acid sequence identity. Human and mouse CLC shares 96% amino acid sequence identity. CLF-1/CLC supports the survival of embryonic motor and sympathetic neurons and has been shown to induce astrocytes differentiation of fetal neuroepithelial cells (4-6). Both CLF-1 and CLC are expressed in the embryo, suggesting that the composite cytokine may have an important role in nervous system development (5, 7). CLC has also been shown to regulate immune functions by stimulating B cell proliferation and Ig production (8).

References

  1. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
  2. Shi, Y. et al. (1999) Biochem. Biophys. Res. Commun. 262:132.
  3. Elson, G.C.A. et al. (1998) J. Immunol. 161: 1371.
  4. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
  5. Elson, G.C.A. et al. (2000) Nature Neurosci. 3:867.
  6. Uemura, A. et al. (2002) Cytokine 18:1.
  7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
  8. Senaldi, G. et al. (2002) J. Immunol. 168:5690.

Entrez Gene IDs

9244 (Human)

Gene Symbol

CRLF1

UniProt

Q9JM58 (CLF-1), Q9QZM3 (CLC)

Additional CLF-1/CLC Complex Products

Product Documents for Recombinant Mouse CLF-1/CLC Complex Protein, CF

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Product Specific Notices for Recombinant Mouse CLF-1/CLC Complex Protein, CF

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