Recombinant Mouse CMG-2 Protein
R&D Systems, part of Bio-Techne | Catalog # 3636-CM
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse CMG-2/ANTXR2 protein
Gly26-Gly318, with a C-terminal 6-His tag
Gly26-Gly318, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly26
Predicted Molecular Mass
32 kDa
SDS-PAGE
32-35 kDa, reducing conditions
Activity
Measured by its ability to bind anthrax protective antigen (PA) in a functional ELISA. Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
Immobilized PA at 1.5 µg/mL (100 µL/well) can bind rmCMG-2 with a linear range of 0.08-5.0 µg/mL.
Immobilized PA at 1.5 µg/mL (100 µL/well) can bind rmCMG-2 with a linear range of 0.08-5.0 µg/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 3636-CM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 3636-CM/CF
| Formulation | Supplied as a 0.2 μm filtered solution in PBS. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CMG-2/ANTXR2
Capillary Morphogenesis Gene-2 (CMG-2) is a widely expressed anthrax toxin receptor (ATR) family protein (1 - 4). CMG-2 is a ~55 kDa protein that contains a 31 amino acid (aa) signal sequence, a 287 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 148 aa cytoplasmic domain. Unlike human CMG-2 which has four isoforms, only one sequence has been reported for mouse CMG-2. The main functional domain of CMG-2 is an extracellular integrin-like von Willebrand factor type A (VWA) domain with a metal ion dependent adhesion site (MIDAS), through which it adheres selectively to collagen type IV and laminin (1 - 6). CMG-2 isoform 2 is induced in HUVEC as they undergo capillary formation in collagen matrices in vitro (4). In humans, CMG-2 is mutated in juvenile hyaline fibromatosis and infantile systemic hyalinosis disorders, and several of these mutations result in loss of laminin binding (7). CMG-2 and the related protein ATR/TEM8 serve as receptors for the protective antigen (PA) of Bacillus anthracis (1, 2). After binding the VWA domain, PA undergoes furin-type cleavage, forms a heptameric receptor/PA pre-pore and binds LF or EF toxin subunits (6, 8, 9). Transport to low pH endosomes, which requires CMG-2 ubiquitination and interaction with the LDL receptor related protein LRP6 (10, 11), allows PA pore formation and release of toxin to the cytoplasm (11, 12). Soluble CMG-2 VWA domain acts as a dummy receptor that can protect cultured cells from anthrax intoxication (2). Within the extracellular region, mouse CMG-2 shares 84%, 91%, 80%, and 83% amino acid sequence homology with human, rat, bovine, and canine CMG-2, respectively. CMG-2 VWA domain also shares 60% aa identity with ATR/TEM8.
References
- Scobie, H.M. and J.A.T. Young (2005), Curr. Opin. Microbiol. 8:106.
- Scobie, H.M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170.
- Swiss Prot. Accession #: Q6DFX2.
- Bell, S.E. et al. (2001) J. Cell Sci. 114:2755.
- Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6367.
- Santelli, E. et al. (2004) Nature 430:905.
- Dowling, O. et al. (2003) Am. J. Hum. Genet. 73:957.
- Wigelsworth, D.J. et al. (2004) J. Biol. Chem. 279:23349.
- Go, M.Y. et al. (2006) J. Mol. Biol. 360:145.
- Abrami, L. et al. (2006) J. Cell Biol. 172:309.
- Wei, W. et al. (2006) Cell 124:1141.
- Lacy, D.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13147.
Long Name
Capillary Morphogenesis Protein 2
Alternate Names
ANTXR2, cI-35, CMG2, ISH, JHS
Gene Symbol
ANTXR2
UniProt
Additional CMG-2/ANTXR2 Products
Product Documents for Recombinant Mouse CMG-2 Protein
Product Specific Notices for Recombinant Mouse CMG-2 Protein
For research use only
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