Recombinant Mouse Coagulation Factor III/Tissue Factor, CF

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 294 amino acid precursor, mouse TF consists of a signal peptide (residues 1 to 28) and the mature chain (residues 29 to 294). As a type I membrane protein, it contains a transmembrane region (residues 252 to 274) and a cytoplasmic tail (residues 275 to 294) (3, 4). The purified rmTF corresponds to the ectodomain (residues 29 to 251) and is potent in activating thermolysin-processed Recombinant Mouse Coagulation Factor VII (Catalog # http://www.rndsystems.com/product_results.aspx?k=3305-SE">3305-SE) under the conditions described in the Activity Assay Protocol.