Recombinant Mouse COCO Protein

R&D Systems, part of Bio-Techne | Catalog # 3356-CC

R&D Systems, part of Bio-Techne
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Carrier Free
3356-CC-025/CF
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With Carrier
3356-CC-025
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Key Product Details

Source

E. coli

Accession #

Q76LW6

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all COCO Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse COCO protein
Arg24-Leu185

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg24

Predicted Molecular Mass

17.3 kDa (monomer)

Activity

Measured by its ability to inhibit BMP-4-induced activity in MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 0.6-3 µg/mL in the presence of 50 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).

Reviewed Applications

Read 1 review rated 5 using 3356-CC in the following applications:

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 3356-CC
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 3356-CC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COCO

COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine knot domain that is homologous to that found in other TGF-beta superfamily ligands such as BMPs that play important roles in tissue morphogenesis and developmental processes (1-6). The mouse COCO cDNA encodes a 185 amino acid (aa) precursor with a 23 aa signal sequence (7, 8). COCO has eight Cys residues in the cysteine knot which places it in the CAN (or eight-membered ring) subfamily of BMP antagonists along with the other DAN family proteins (1). Mature mouse COCO shares 62% and 27% aa sequence identity with human and Xenopus COCO, respectively. It shares 22%-27% aa sequence identity with mouse DAN, Gremlin, PRDC, and Cerberus. In Xenopus embryos, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO is required for Xenopus left-right axis formation (9). It functions predominantly on the right side of the embryo, although it is equally expressed on both left and right sides (9). COCO binds and inhibits activin, BMP-4, GDF-3/derrière, Wnt8, and Xnr1 (7, 9). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8).

References

  1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
  2. Katoh, M. and M. Katoh (2004) Oncol. Rep. 12:423.
  3. Katoh, M. and M. Katoh (2005) Int. J. Mol. Med. 15:885.
  4. Baron, M.H. (2005) Exp. Hematol. 33:1015.
  5. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  6. De Robertis, E.M. and H. Koruda (2004) Annu. Rev. Cell Dev. Biol. 20:285.
  7. Bell, E. et al. (2003) Development 130:1381.
  8. Pearce, J.J. et al. (1999) Dev. Biol. 209:98.
  9. Vonica, A. and A.H. Brivanlou (2007) Dev. Biol. 303:281.

Alternate Names

Cerberus 2, Cerberus like-2, CKTSF1B3, DAND5, Dante, GREM3

Entrez Gene IDs

199699 (Human); 23863 (Mouse); 685719 (Rat)

Gene Symbol

DAND5

UniProt

Q76LW6

Additional COCO Products

Product Documents for Recombinant Mouse COCO Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse COCO Protein

For research use only

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