Recombinant Mouse CXCL10/IP-10/CRG-2 Protein
R&D Systems, part of Bio-Techne | Catalog # 466-CR
Key Product Details
Product Specifications
Source
E. coli-derived mouse CXCL10/IP-10/CRG-2 protein
Ile22-Pro98, with an N-terminal Met
Ile22-Pro98, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
8.8 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR3.
The ED50 for this effect is 0.05-0.3 µg/mL.
The ED50 for this effect is 0.05-0.3 µg/mL.
Reviewed Applications
Read 1 review rated 4 using 466-CR in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 466-CR
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 466-CR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CXCL10/IP-10/CRG-2
The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha/ beta/ gamma-interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.
References
- Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
- Vanguri, P. (1996) J. Neuroimmunol. 56:35.
- Sgadari, C. et al. (1996) Blood, 87:3877.
Alternate Names
CRG-2, CRG2, IP-10
Gene Symbol
CXCL10
UniProt
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Recombinant Mouse CXCL10/IP-10/CRG-2 Protein
Product Specific Notices for Recombinant Mouse CXCL10/IP-10/CRG-2 Protein
For research use only
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