Recombinant Mouse CXCL11/I-TAC Protein
R&D Systems, part of Bio-Techne | Catalog # 572-MC
Key Product Details
Product Specifications
Source
E. coli-derived mouse CXCL11/I-TAC protein
Phe22-Met100
Phe22-Met100
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Phe22
Predicted Molecular Mass
9.1 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR3.
The ED50 for this effect is 3-15 ng/mL.
The ED50 for this effect is 3-15 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 572-MC
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 572-MC/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CXCL11/I-TAC
CXCL11, also known as I-TAC, SCYB9B, H174, IP-9, and beta-R1, is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to yield a 79 aa residue mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (Crg-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid.
References
- Widney, D.P. et al. (2000) J. Immunol. 164:6322.
- Meyer, M. et al. (2000) Cytogenet. Cell Genet. 88:278.
- Loetscher, P. et al. (2001) J. Biol. Chem. Manuscript M005652200.
Alternate Names
beta-R1, H174, I-TAC, ITAC, SCYB9B
Gene Symbol
CXCL11
UniProt
Additional CXCL11/I-TAC Products
Product Documents for Recombinant Mouse CXCL11/I-TAC Protein
Product Specific Notices for Recombinant Mouse CXCL11/I-TAC Protein
For research use only
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