Recombinant Mouse Cystatin F Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4557-PI
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Cystatin F protein
Ala19-Gln144, with a C-terminal 6-His tag
Accession # O89098
Ala19-Gln144, with a C-terminal 6-His tag
Accession # O89098
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala19
Predicted Molecular Mass
15 kDa
SDS-PAGE
20 kDa, reducing conditions
Activity
Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 value is <60 nM, as measured under the described conditions.
The IC50 value is <60 nM, as measured under the described conditions.
Formulation, Preparation and Storage
4557-PI
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cystatin F
Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a new member of the Cystatin superfamily (1-3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards Cathepsin L and legumain (1, 2, 5). Compared to other secreted Cystatins including C, D, E/M, S, SA and SN, which contain two intrachain disulfide bonds, Cystatin F has two extra Cys residues that may be involved in interchain disulfide bonds. rmCystatin F and rhCystatin F (Catalog # http://www.rndsystems.com/product_results.aspx?k=1889-PI">1889‑PI) exhibit disulfide bond-linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human Cystatin F and glutathione S-transferase (2).
References
- Ni, J. et al. (1998) J. Biol. Chem. 273:24797.
- Halfon, S. et al. (1998) J. Biol. Chem. 273:16400.
- Utsunomiya, T. et al. (2002) Clin. Cancer 8:2591.
- Werle, B. et al. (2003) Biol. Chem. 384:281.
- Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
Alternate Names
CMAP, CST7, Cystatin 7, Leukocystatin
Entrez Gene IDs
8530 (Human)
Gene Symbol
CST7
UniProt
Additional Cystatin F Products
Product Documents for Recombinant Mouse Cystatin F Protein, CF
Product Specific Notices for Recombinant Mouse Cystatin F Protein, CF
For research use only
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