Recombinant Mouse DC-SIGN/CD209 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8345-DC

R&D Systems, part of Bio-Techne
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8345-DC-050
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Key Product Details

Source

NS0

Accession #

Q91ZX1

Conjugate

Unconjugated

Applications

Bioactivity

View all DC-SIGN/CD209 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse DC-SIGN/CD209 protein
Val73-Lys238, with an N-terminal HA tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr (HA tag)

Predicted Molecular Mass

20 kDa

SDS-PAGE

21-26 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse DC-SIGN is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse ICAM-5 Fc Chimera (Catalog # 1173-M5) that produces 50% optimal binding response is 0.8-4 μg/mL.

Formulation, Preparation and Storage

8345-DC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-SIGN/CD209

Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).

References

  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Caminschi, I. et al. (2001) Mol. Immunol. 38:365.
  3. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

Alternate Names

CD209, CLEC4L, DC-SIGN1, DCSIGN

Entrez Gene IDs

30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)

Gene Symbol

CD209

UniProt

Q91ZX1

Additional DC-SIGN/CD209 Products

Product Documents for Recombinant Mouse DC-SIGN/CD209 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse DC-SIGN/CD209 Protein, CF

For research use only

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