Recombinant Mouse EOGT Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8116-GT

R&D Systems, part of Bio-Techne
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8116-GT-020
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Key Product Details

Source

NS0

Accession #

Q8BYW9

Conjugate

Unconjugated

Applications

Enzyme Activity

View all EOGT Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse EOGT protein
His23-Lys523, with C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His23

Predicted Molecular Mass

59 kDa

SDS-PAGE

57-61 kDa, reducing conditions

Activity

Measured by its ability to transfer GlcNAc from UDP-GlcNAc to Recombinant Drosophila Notch EGF20 (Catalog # 8117-DN).
The specific activity is >0.7 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8116-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: EOGT

O-GlcNAc (O-linked beta-N-acetylglucosamine) results from the addition of a single N-acetyl-glucosamine residue to serine/threonine residues. O-GlcNAc is involved in many cellular processes such as nutrient sensing, stress response, transcription, translation, cell signaling and cell cycle regulation (1). It is also involved in many human diseases including diabetes, Alzheimer’s disease and cancer (2). O-GlcNAc shares many features with protein phosphorylation, a fundamental mechanism for intracellular communication (3, 4). The modification occurs on nuclear and cytosolic proteins (3), and has been recently discovered on extracellular proteins (5). Unlike other types of glycosylation, O-GlcNAc is not elongated into complex oligosaccharides. O-GlcNAc is introduced by a single intracellular O-GlcNAc transferase (OGT) (6) and a single extracellular O-GlcNAc transferase (EOGT) (7), and removed by O-GlcNAcase (OGA) (8). EOGT is involved in the regulation of Notch receptor (9). Mutation of EOGT results in autosomal recessive Adams-Oliver syndrome (10, 11). The enzymatic activity of recombinant mouse EOGT was determined using a phosphatase-coupled assay (12).

References

  1. Hart, G. W. et al. (2007) Nature, 446:1017.
  2. Slawson, C., et al. (2006) J. Cell Biochem. 97:71.
  3. Comer, F. I. and Hart, G. W. (2001), Biochemistry 40:7845.
  4. Love, D. C. and Hanover, J. A. (2005), Sci. STKE 2005: re13.
  5. Sakaidani, Y. et al. (2011) Nat. Commun. 2:583.
  6. Haltiwanger, R. S. et al. (1990) J. Biol. Chem. 265:2563.
  7. Sakaidani, Y. et al. (2012), Biochem. Biophys. Res. Commun. 419:14.
  8. Gao, Y. et al. (2001), J. Biol. Chem. 276: 9838-45.
  9. Muller, R. et al. (2013) PLoS One 8:e62835.
  10. Cohen, I. et al. (2013) Eur. J. Hum. Genet. Online publication ahead of print.
  11. Shaheen, R. et al. (2013) Am. J. Hum. Genet. 92 :598.
  12. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

EGF Domain-specific O-linked N-acetylglucosamine (GlcNAc) Transferase

Alternate Names

AER61, C3orf64

Entrez Gene IDs

285203 (Human); 101351 (Mouse)

Gene Symbol

EOGT

UniProt

Q8BYW9

Additional EOGT Products

Product Documents for Recombinant Mouse EOGT Protein, CF

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Product Specific Notices for Recombinant Mouse EOGT Protein, CF

For research use only

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