Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 602-A1

R&D Systems, part of Bio-Techne
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602-A1-200
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Key Product Details

Source

NS0

Accession #

P52793

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all Ephrin-A1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-A1 protein
Mouse Ephrin-A1
(Asp19 - Ser182)
Accession # P52793		 IEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp19

Predicted Molecular Mass

46.8 kDa (monomer)

SDS-PAGE

50-55 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rmEphA2/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A1 Fc Chimera with a linear range of 0.16-10 ng/mL.

Formulation, Preparation and Storage

602-A1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A1

Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Mouse Ephrin-A1 is synthesized with an 17 amino acid (aa) signal peptide, a 165 aa mature chain, and a 23 aa C‑terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane (3, 4). It can also be released as a soluble molecule (3, 5, 6). The mature 21 ‑ 25 kDa mouse Ephrin-A1 shares 85% and 94% aa sequence identity with human and rat Ephrin-A1, respectively. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin (4, 8). It is expressed on resting CD4+ T cells but is down‑regulated following activation (7, 8). Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis (7, 8). In cancer, Ephrin‑A1 is expressed by tumor cells as well as on the tumor-associated vasculature (5, 6, 9). It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis (10 - 12). Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension (13).

References

  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Takahashi, H. and T. Ikeda (1995) Oncogene 11:879.
  4. Shao, H. et al. (1995) J. Biol. Chem. 270:5636.
  5. Easty, D.J. et al. (1995) Cancer Res. 55:2528.
  6. Cui, X.-D. et al. (2010) Int. J. Cancer 126:940.
  7. Wohlfahrt, J.G. et al. (2004) J. Immunol. 172:843.
  8. Aasheim, H.-C. et al. (2005) Blood 105:2869.
  9. Ogawa, K. et al. (2000) Oncogene 19:6043.
  10. Liu, D.-P. et al. (2007) Int. J. Oncol. 30:865.
  11. Brantley-Sieders, D.M. et al. (2006) Cancer Res. 66:10315.
  12. Pandey, A. et al. (1995) Science 268:567.
  13. Magal, E. et al. (1996) J. Neurosci. Res. 43:735.

Alternate Names

B61, EFL-1, EFNA1, EphrinA1, LERK1

Entrez Gene IDs

1942 (Human); 13636 (Mouse)

Gene Symbol

EFNA1

UniProt

P52793

Additional Ephrin-A1 Products

Product Documents for Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

For research use only

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