Recombinant Mouse Ephrin-A3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7395-EA

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

NP_034238

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Ephrin-A3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-A3 protein
Mouse Ephrin-A3
(Gln23-Gly206)
Accession # NP_034238		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln23 predicted: No results obtained, sequencing might be blocked

Predicted Molecular Mass

48 kDa (monomer)

SDS-PAGE

60-66 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of PC-3 human prostate cancer cells.
The ED50 for this effect is 15-75 ng/mL.

Formulation, Preparation and Storage

7395-EA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A3

Ephrin‑A3, also known as EHK1‑L, EFL‑2, and LERK‑3, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Ephrin‑A3 preferentially interacts with receptors in the EphA family (1, 2). Ephrin‑A3 is an unusual Ephrin‑A molecule in its dependence on heparan sulfate binding for full activity (3). Mature mouse Ephrin‑A3 shares 92% and 100% aa sequence identity with human and rat Ephrin‑A3, respectively (4). Its expression is restricted to discreet locations during the early development of multiple tissues (5). Ephrin‑A3 expression can be up‑ or down‑regulated by hypoxia in the hippocampus or vascular endothelial cells, respectively (6, 7). Ephrin‑A3 down‑regulation contributes to hypoxia‑induced endothelial cell chemotaxis, proliferation, and tubule formation (7). Its interaction with EphA receptors induces neurite growth cone collapse and the repulsion of migrating axons (8‑10). This activity is important for the accurate pathfinding of migrating axons during CNS development (10). Astrocyte‑expressed Ephrin‑A3 activates EphA4 on hippocampal neurons to regulate dendritic spine morphology and long term potentiation (8, 11, 12). The same interaction induces reverse signaling through Ephrin‑A3 to regulate glutamate uptake by the astrocyte and the availability of glutamate in the synapse (11, 12). Astrocyte‑expressed Ephrin‑A3 also interacts with EphA7 to inhibit the proliferation of neural progenitor cells (13).

References

  1. Pasquale, E.B. (2008) Cell 133:38.
  2. Astin, J.W. et al. (2010) Nat. Cell Biol. 12:1194
  3. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  4. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  5. Irie, F. et al. (2008) Proc. Natl. Acad. Sci. 105:12307.
  6. Ceretti, D.P. and N. Nelson (1998) Genomics 47:131.
  7. Duffy, S.L. et al. (2006) Gene Expr. Patterns 6:719.
  8. Pulkkinen, K. et al. (2008) FEBS Lett. 582:2397.
  9. Fasanaro, P. et al. (2008) J. Biol. Chem. 283:15878.
  10. Murai, K.K. et al. (2003) Nat. Neurosci. 6:153.
  11. Stein, E. et al. (1999) J. Neurosci. 19:8885.
  12. Rudolph, J. et al. (2010) Cell Adh. Migr. 4:400.
  13. Filosa, A. et al. (2009) Nat. Neurosci. 12:1285.
  14. Carmona, M.A. et al. (2009) Proc. Natl. Acad. Sci. 106:12524.
  15. Jiao, J. et al. (2008) Proc. Natl. Acad. Sci. 105:8778.

Alternate Names

EFL-2, EFNA3, Ehk1-L, EphrinA3, LERK3

Entrez Gene IDs

1944 (Human)

Gene Symbol

EFNA3

UniProt

NP_034238

Additional Ephrin-A3 Products

Product Documents for Recombinant Mouse Ephrin-A3 Fc Chimera Protein, CF

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