Recombinant Mouse F-Spondin/SPON1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7950-SP

R&D Systems, part of Bio-Techne
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7950-SP-050
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Key Product Details

Source

NS0

Accession #

Q8VCC9

Conjugate

Unconjugated

Applications

Bioactivity

View all F-Spondin/SPON1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse F-Spondin/SPON1 protein
Leu27-Cys807

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu27

Predicted Molecular Mass

88.2 kDa

SDS-PAGE

105-115 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Able to significantly enhance neurite outgrowth when immobilized at 1.25‑5.0 μg/mL.

Formulation, Preparation and Storage

7950-SP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
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Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: F-Spondin/SPON1

F‑Spondin (floor plate and thrombospondin homology), also called Spondin‑1, SPON1 or VSGP (vascular smooth muscle growth‑promoting factor), is an approximately 110 kDa secreted glycoprotein that is a member of a subgroup of TSR (thrombospondin) molecules that are either membrane‑bound or associated with the extracellular matrix (ECM) (1‑3). Mouse F‑Spondin is synthesized as an 807 amino acid (aa) precursor with a 779 aa mature region that includes an N‑terminal reelin‑like domain, an F‑spondin (FS) domain, and six C‑terminal thrombospondin (TSP) type I repeats (1‑3). Mature mouse F‑Spondin shares 99% aa sequence identity with rat and 97% with human, bovine and canine F‑Spondin. TSP 5 and 6 bind ECM, while TSP 1‑4 plus the FS domain may mediate repulsive activity on motor neurons and outgrowth promoting activity on sensory neurons during development or after injury (2‑5). Crystal structure indicates that the reelin‑like domain binds heparin and may mediate weak dimerization (6). Plasmin cleavage generates a diffusible 95 kDa, 656 aa F‑spondin that lacks TSP 5 and 6, while non‑plasmin cleavage between the FS segment and the first TSP repeat generates 60 kDa and 50 kDa fragments (3, 4, 7). F‑Spondin shows unusual C‑mannosylation and O‑fucosylation within the TSP repeats (3). Mammalian cells expressing F‑spondin include floor plate epithelium, ventral motor neurons, Schwann cells, fibroblasts, hippocampal pyramidal cells, endothelial cells, vascular smooth muscle cells and some tumor cells (2‑5, 8). F‑Spondin can either tether cells to the ECM or interfere with integrin adhesion, thus either blocking or allowing nerve or vascular endothelial cell migration (3, 9). It binds beta‑amyloid fibrils and inhibits beta‑secretase cleavage, thus reducing A beta plaque deposition associated with Alzheimer’s disease (10, 11). F‑Spondin is also reported to inhibit differentiation or migration during angiogenesis (affecting endothelial cells) and bone development (affecting osteoclast and chondrocyte precursors) (3, 9, 12, 13).

References

  1. Miyamoto, K. et al. (2001) Arch. Biochem. Biophys. 390:93.
  2. Klar, A. et al. (1992) Cell 69:95.
  3. Feinstein, Y. and A. Klar (2004) Int. J. Biochem. Cell Biol. 36:975.
  4. Burstyn-Cohen, T. et al. (1998) J. Neurosci. 18:8875.
  5. Feinstein, Y. et al. (1999) Development 126:3637.
  6. Tan, K. et al. (2008) J. Mol. Biol. 381:1213.
  7. Tzarfaty-Majar, V. et al. (2001) J. Biol. Chem. 276:28233.
  8. Pyle-Chenault, R.A. et al. (2005) Tumor Biol. 26:245.
  9. Terai, Y. et al. (2001) J. Cell Physiol. 188:394.
  10. Ho, A. and T.C. Sudhof (2004) Proc. Natl. Acad. Sci. USA 101:2548.
  11. Hafez, D.M. et al. (2012) Neuroscience 223:465.
  12. Palmer, G.D. et al. (2010) J. Orthop. Res. 28:1323.
  13. Oka, H. et al. (2011) J. Periodontol. 82:1776.

Alternate Names

FSpondin, SPON1, VSGP

Entrez Gene IDs

10418 (Human); 233744 (Mouse); 64456 (Rat)

Gene Symbol

SPON1

UniProt

Q8VCC9

Additional F-Spondin/SPON1 Products

Product Documents for Recombinant Mouse F-Spondin/SPON1 Protein, CF

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Product Specific Notices for Recombinant Mouse F-Spondin/SPON1 Protein, CF

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