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Recombinant Mouse FABP1/L-FABP Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9467-CL

R&D Systems, part of Bio-Techne
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9467-CL-100

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Product Specifications

Source

E. coli-derived mouse FABP1/L-FABP protein
Met1-Ile127

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

14 kDa

SDS-PAGE

12 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9467-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

Fatty acid binding proteins (FABP) are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner and are involved in intracellular lipid transport. All FABPs bind free fatty acids, cholesterol, and retinoids, which differ in their selectivity, affinity and binding mechanism (1). Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis (2). FABPs are structurally conserved, consisting of a water-filled, ligand-binding pocket surrounded by ten anti-parallel beta-barrel structures, capped by an N-terminal helix-turn-helix motif. The helical N-terminus is involved in the regulation of FA transfer from membranes (3).FABP1, also known as liver FABP (L-FABP) is highly expressed in the liver, intestine, kidney and lung (1). FABP1 binds free fatty acids and their co-enzyme A derivatives. FABP1 is unique among other members in FABP family, attributed to its ability to bind multiple ligands at once. It has a larger solvent-accessible core in comparison to other FABPs, and this allows for more diverse binding to substrates (1). Mouse FABP1 is 127 amino acids (aa) in length. It is a two beta-sheet molecule that contains an acetylated initiating methionine. Full-length mouse FABP1 shares 94% and 84% aa identity with rat and human FABP1, respectively (4).

References

  1. Smathers, R. et al. (2011) Hum. Genomics. 5(3):170.
  2. Furuhashi, M. et al. (2008) Nat. Rev. Drug Discov. 7(6):489.
  3. Storch, J. et al. (2010) J. Biol. Chem. 285(43):32679.
  4. Martin, G. et al. (2013) Biochemistry 52(51):9347.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

UniProt

Additional FABP1/L-FABP Products

Product Documents for Recombinant Mouse FABP1/L-FABP Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse FABP1/L-FABP Protein, CF

For research use only

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