Recombinant Mouse FABP4/A-FABP Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9480-CL

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

P04117

Conjugate

Unconjugated

View all FABP4/A-FABP Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse FABP4/A-FABP protein
MHHHHHH GS  Mouse FABP4/A-FABP
(Met1-Ala132)
Accession # P04117
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

14 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9480-CL
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 1 mg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP4/A-FABP

Fatty acid binding protein-4 (FABP4; also named A- (adipocyte) FABP, ALBP, or adipocyte protein 2 (aP2)) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1, 5, 6). FABP4 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length (1, 2, 3). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. The FABP family members are subdivided based on organ or tissue type it was originally expressed or identified; liver- (L-FABP), intestine- (I-FABP), heart- (H-FABP), adipocyte- (A-FABP), epidermal- (E-FABP), ileal- (Il-FABP), brain- (B-FABP), myelin- (M-FABP) and testis-FABP (T-FABP) (1). Mouse A- FABP, the product of the FABP4 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure generated by a series of antiparallel beta -strands and two alpha ‑helices (4, 8). FABP4 is able to bind long-chain fatty acids, retinoic acids or eicosanoids with one mol of ligand bound per mole of protein (1, 8, 9). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand (4, 7, 9). Mouse FABP4 is 92%, 94% and 91% aa identical to human, rat and canine FABP4, respectively. It also shows 26% and 28% aa identity to mouse L-FABP and I‑FABP, respectively.

References

  1. Smathers, R & Petersen, D. (2011) Human Genomics 5:170.
  2. Storch, J. & Thumser, AE. (2000) Biochim Biophys Acta. 1486:28.
  3. Zimmerman, A.W. & Veerkamp, J.H. (2007) Protein Sci. 9:2042.
  4. Bernlohr, D. et al. (1997) Ann. Rev. of Nut. 17:277.
  5. Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
  6. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
  7. Xu Z. et. al. (1992) Biochemistry. 31:3484.
  8. Matarese, V. & Bernlohr, D. (1988) J Biol Chem. 263:14544.
  9. Majava,V. et al. (2010) PLoS One. 5:e10300.

Long Name

Fatty Acid-Binding Protein 4

Alternate Names

A-FABP, AFABP, aP2

Entrez Gene IDs

2167 (Human); 11770 (Mouse); 79451 (Rat)

Gene Symbol

FABP4

UniProt

P04117

Additional FABP4/A-FABP Products

Product Documents for Recombinant Mouse FABP4/A-FABP Protein, CF

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Product Specific Notices for Recombinant Mouse FABP4/A-FABP Protein, CF

For research use only

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