Recombinant Mouse Fetuin A/AHSG Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1563-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Fetuin A/AHSG protein
Ala19-Ile345, with a C-terminal 10-His tag
Ala19-Ile345, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala19
Predicted Molecular Mass
37 kDa
SDS-PAGE
58 kDa, reducing conditions
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <0.20 µM, as measured under the described conditions.
The IC50 value is <0.20 µM, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 1563-PI in the following applications:
Formulation, Preparation and Storage
1563-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Fetuin A/AHSG
Mouse Fetuin A, also known as alpha2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It has been also called "countertrypsin" because of its ability to inhibit trypsin (1). It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (2, 3). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (4). The major form of plasma Fetuin A corresponds to two disulfide bond-linked chains derived from the single chain (5). Fetuin-A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300-600 μg/mL), which fall significantly (30-50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (7, 8).
References
- Yamamoto, K. and H. Sinohara (1993) J. Biol. Chem. 268:17750.
- Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
- Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
- Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
- Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta 671:78.
- Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
- Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
- Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
Long Name
alpha-2-HS Glycoprotein
Alternate Names
AHSG, alpha-2-HS-glycoprotein
Gene Symbol
AHSG
UniProt
Additional Fetuin A/AHSG Products
Product Documents for Recombinant Mouse Fetuin A/AHSG Protein, CF
Product Specific Notices for Recombinant Mouse Fetuin A/AHSG Protein, CF
For research use only
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